I. Precautions and protective measures for catching animals
1. When catching experimental animals, first of all, we should pay attention to appease the animals, and don't yell, make a scene, poke at random and other behaviors that are easy to irritate the animals.
2. Pay attention to the correct methods to catch experimental animals, such as rabbits scratching the skin at the back of their necks, scratching their buttocks, and not scratching their ears. Rats can't just scratch their tails, so they are easily attacked by their reflexes.
If experimental animals are not docile or aggressive, we should pay attention to protective measures. Such as protective gloves, protective clothing, etc. If the experimental animal has been angered, it can be calmed down a little and then arrested.
Second, anesthesia (general anesthesia, local anesthesia) matters needing attention
1. Dose control should be paid attention to during general anesthesia to avoid overdose.
2. When injecting general anesthesia drugs, you should inject them from the far end of the ear vein, hold the rabbit ear tightly, and beware of breaking free.
3. When injecting general anesthesia drugs, always observe whether it is injected into the vein. If the propulsion is blocked, observe whether the surrounding tissue is swollen, adjust the needle and then inject.
4. The injection of general anesthesia drugs should be fast first and then slow, and the injection should be carried out while observing the signs. After the anesthetic effect is achieved, the administration can be stopped.
5. If the animal struggles violently during local operation, local anesthesia can be added, which acts on the mucosa.
Three. Matters needing attention in organizational separation
1. should be separated layer by layer, first with a knife or scissors to separate the skin fascia, etc. Then use blunt objects such as hemostatic forceps to separate subcutaneous tissue, muscle fascia, etc.
2. Pay attention to the separation of blood vessels and nerves when separating, and don't pull it too much to prevent it from breaking.
3. Normal tissue space can be selected for separation, so as to avoid damaging other tissues such as blood vessels, which can make the visual field clean and facilitate subsequent operation.
4. Muscle separation should be blunted along the fiber direction. If crosscutting is needed, hemostatic measures should also be taken.
Four, trachea separation matters needing attention
1. Make full preparations before the experiment to predict the position of trachea.
2. Separate the tissue layer by layer, and gently separate the soft tissue behind the trachea after the trachea is visible to avoid tracheal congestion.
3. The separation process should not be rough, so as not to cause unnecessary damage to trachea and other tissues.
Five, arterial intubation matters needing attention and countermeasures
1. The premise of arterial intubation is to find the artery accurately, so we should carefully distinguish the artery, vein and other tissues to avoid inserting it in the wrong place.
2. The arteries should be carefully separated before intubation, especially the nerves accompanying the arteries, so as not to damage the nerves.
3. Don't be rude when separating the artery, and don't pull the artery excessively, so as not to cause arterial injury or stress reaction to affect blood pressure monitoring.
4. Before intubation, the distal artery should be carefully ligated, not too loose. If there is still oozing blood after ligation, it should be ligated again.
5. Proximal artery should be clamped by arterial clamp, and hemostatic forceps should not be used to avoid damaging artery.
6. Use ophthalmic scissors to cut an oblique orifice of the artery, and it is appropriate to use 1/2 of the vascular caliber. If it is too small, it is difficult to insert the cannula, and if it is too large, it will easily cause blood vessel rupture.
7. The incision should be as close as possible to the distal end of the artery to prevent the experiment from failing, and then make incision remedy.
8. Due to the high pressure in the artery, blood will overflow when it is cut. If only a small amount of blood overflows after incision, it is necessary to consider whether the tissue is an artery or the incision is too small.
9. Intubation should not be rude, so as not to puncture the blood vessel wall and cause injury and bleeding.
10. The insertion length of the cannula should not be too short, otherwise it will be difficult to fix and easy to detach.
1 1. If blood pressure cannot be detected after normal insertion, it should be checked whether it is inserted into blood vessels, not other tissues outside blood vessels.
12. If there is massive bleeding, clamp the hemostasis point with artery in time, and don't panic.