1, the technical development is lagging behind. At present, DNA extraction technology is still stuck in Chelex- 100 method. Conventional methods such as organic extraction have many defects, such as impure DNA extraction, time-consuming, low efficiency, inability to extract quantitatively, and low success rate of amplification after DNA extraction.
2. Lack of independent intellectual property rights. Biological companies in the United States, Germany, Norway, Finland and other countries have developed new DNA extraction kits for many years, which have successfully solved many problems such as DNA extraction, purification and quantitative extraction, especially the technology that magnetic beads can realize automatic extraction is more attractive. However, because these kits are based on patented technology, they are expensive and cannot be popularized in China. It is even more unattainable to apply it to the extraction of a large number of biological samples needed to establish a DNA database.