step
First, genetic engineering.
Second, the basic steps of genetic engineering pharmacy
Third, the specific steps of genetic engineering pharmacy
Section 2 Cell Engineering Pharmacy
I. Cell engineering
Second, cell engineering pharmacy
Section 3 Enzyme Engineering Pharmacy
I. Enzymes and enzyme engineering
Second, enzyme engineering pharmacy
Section 4 Fermentation Engineering Pharmacy
I. Fermentation and Fermentation Engineering
Second, fermentation engineering pharmacy
Section 5 protein Engineering Pharmacy
I. protein and protein projects
Second, protein Engineering Pharmaceutical
Section 6 Biomedical Engineering and Biological Information
engineering
I. Biomedical engineering
Second, bioinformatics engineering
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Chapter II Gene Cloning and Expression Genetic Engineering Pharmacy 1
I. Escherichia coli expression system
Second, the yeast expression system
Three, insect cell expression system
In the second quarter, the manifestations of various products adopted
Separation and purification method
I. Intracellular insoluble expression products of Escherichia coli
Secondly, the expression product is secreted.
Three. Soluble expression products in Escherichia coli cells
4. Expression of protein in periplasm of Escherichia coli cells.
Section III Activity and Safety of Expressed Products
Evaluation and stability study
A, biological activity (titer) determination
Second, the safety evaluation
Third, stability.
Fourth, products.
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Chapter III Cell Engineering Pharmacy Section 1 Cell, Cell Engineering and Cell
Engineering pharmacy
I. Cells
Second, cell engineering.
Third, cell engineering pharmacy
Section 2 Animal Cell Engineering Pharmacy
I. totipotency of animal cells
Second, the application of transgene in animal cell engineering pharmacy
And cell culture technology.
Section 3 Plant Cell Engineering Pharmacy
First, the characteristics of plant cells
Second, the research of plant cell engineering drugs
Three, plant cell fusion and nuclear transfer technology
Four, transgenic plant drug technology
Five, plant cell culture engineering
Six, plant cell chromosome engineering
Section IV Application and Prospect of Cell Engineering Pharmacy
And the existing problems.
I. Cell engineering drugs for clinical use
Two, humanized antibody and the development of "molecular medicine"
engineering
Third, the safety of cell engineering drugs
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Chapter IV Overview of Enzyme Engineering Pharmacy Section 1
I. Enzymes and their catalytic characteristics
Second, the main factors affecting the enzymatic reaction
Third, the classification of enzymes.
Four, enzyme engineering and enzyme engineering pharmacy
Section 2 Enzyme Engineering Pharmaceutical Technology
First, the production technology of medicinal enzymes
Second, the enzymatic production technology of drugs
Section III Current Situation and Prospect of Enzyme Engineering Pharmacy
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Chapter V Fermentation Engineering Pharmacy Section 1 Fundamentals of Fermentation Engineering Pharmacy
I. Drugs produced by microbial fermentation
Second, microorganisms in the fermentation pharmaceutical industry
The second quarter microbial drug fermentation process
Section 3 Control of Microbial Fermentation Process
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Chapter VI The concept of protein Engineering Pharmaceutical 1 protein Engineering Pharmaceutical.
First, the core content of engineering pharmaceutical research is in protein
And the basic concepts and methods.
Second, some ways to transform protein.
In the second quarter, protein engineering pharmaceutical methods
First, random mutation
Second, in vitro recombination.
Three. The Common Purpose of protein's Engineering Pharmaceutical Methods
Section III Status of Engineering Drugs in protein
I. Site-directed Mutation Transformation in protein
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refer to
Chapter VII Nucleic Acid Drugs Section 1 Antisense Nucleic Acid Drugs
First, the mechanism of antisense drugs
Second, the design strategy of antisense drugs
Third, the characteristics of antisense drugs
Synthesis and purification of antisense nucleic acid drugs
Section 2 short interfering RNA(siRNA)
First, the mechanism of RNAi
Second, the characteristics of RNAi
Third, the preparation method of siRNA
Section 3 miRNA
First, the basic characteristics of miRNA
Second, the mechanism of miRNA.
Three. Similarities and differences between siRNA and miRNA
Section IV Current Situation of Clinical Trials of Nucleic Acid Drugs
And prospects
First, the current situation of clinical trials of antisense nucleic acid drugs
And prospects
Second, the present situation and prospect of clinical trials of RNAi
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Chapter VIII Polypeptide Drugs Section 1 Concept and advantages of polypeptide drugs
First, the concept of polypeptide and polypeptide drug
Second, the advantages of polypeptide drugs
Section 2 Preparation of Polypeptide Drugs
First, the solid phase synthesis method
Second, the liquid phase synthesis method
Third, the polypeptide synthesis method
Fourthly, preparing polypeptide by enzymolysis.
In the third quarter, stability, testing,
Preparation and route of administration
First, the stability of polypeptide drugs
Second, the analysis method of polypeptide pharmaceutical preparations
Thirdly, the study of sustained-release preparation of polypeptide drugs.
Four. Administration route of polypeptide drugs
Section IV Application of Polypeptide Drugs
I. Polypeptide vaccine
Second, anti-tumor peptides.
Third, antiviral peptides.
Fourthly, polypeptide-directed drugs.
Verb (abbreviation of verb) antibacterial active peptide
Six, cytokine mimic peptide
Polypeptides for cardiovascular diseases
Eight, other medicinal small peptides
Nine, diagnostic peptides
Think about a problem
refer to
Chapter IX Overview of Therapeutic Antibody Drugs Section 1 Antibodies
First, the basic structure of antibody
Second, the genetic composition of antibodies.
Thirdly, the generation of antigen recognition specificity
Four. Affinity and binding force
Verb (abbreviation for verb) category conversion
Pharmacokinetics of intransitive verbs
Seven, the effect function
Section 2 Micro-antibodies for Treatment
Section 3 Single Chain Antibodies and Single Domain Antibodies
Section 4 Recombinant human-mouse chimeric monoclonal antibody
Section 5 Recombinant humanized monoclonal antibody
I. human monoclonal antibody
Section 6 Antibody Drugs and Chemotherapy Drugs
Combined application
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ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ ⅸ 856
therapy
Section 2 dendritic cells * * * cultured cells
factor
Section III Therapeutic Cloning
Section 4 Nuclear Transplantation and Reprogramming
refer to
1 1 Chapter Cytokine Drugs Section 1 Recombinant human interferon
I. Overview
Second, the nature, structure and function of interferon
Three. Biological Activity and Mechanism of Interferon
4. Preparation of Interferon by Genetic Engineering
Verb (abbreviation of verb) Interferon's Clinical Application and Adverse Reactions
Section II Recombinant Human Interleukin
I. Overview
Second, all kinds of IL
Section III Granulocyte Colony Stimulating Factor
I. Structure and nature
Second, g? CSF、GM？ Clinical application of cerebrospinal fluid
Third, g? CSF、GM？ Adverse reaction of cerebrospinal fluid
4. Recombinant human G? CSF and GM? Cerebrospinal Fluid
Section 4 Erythropoietin
I. Gene and molecular structure of erythropoietin
Second, the production and properties of erythropoietin
3. Preparation of recombinant human erythropoietin
Fourth, clinical application
Adverse reaction of verb (abbreviation of verb) to erythropoietin
Section 5 Tissue Plasminogen Activator
I. Overview
Second, the nature, structure and function
Three. Biological activity and mechanism of action
Fourth, genetic engineering preparation t? Panama
Verb (abbreviation of verb) recombines t? Clinical application of PA
Section VI Tumor Necrosis Factor
I. Overview
Second, the molecular structure and function of TNF
Three. Biological activity and clinical application of tumor necrosis factor
Fourthly, the preparation of TNF
Think about a problem
refer to
Chapter 12 Gene Therapy Section 1 Research Status of Gene Therapy
First, the status quo of gene therapy
Second, the current problems of gene therapy
Section 2 Methods of Gene Transfer
First, the concept and steps of gene therapy
Second, gene transfer methods.
Section III Application and Safety of Gene Therapy
First, the application of gene therapy
Second, the safety of gene therapy.
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refer to
Chapter 13 Animal Genetic Engineering Drugs Section 1 Animal Cell Culture
First, several basic concepts of animal cell culture
Second, the basic conditions of animal cell culture
Three, animal cell morphology
Four. Cryopreservation of mammalian cells
Verb (abbreviation for verb) thawing and resuscitation of animal cells
Sixth, subculture of animal cells.
Section 2 Animal Transgenic Technology
I. Transgenic technology of animal cells
Second, the preparation technology of transgenic animals
Section 3 Animal Reactors and Animal Genes
Engineering drugs
A, transgenic animal cell bioreactor
Second, the mammary gland bioreactor
Think about a problem
refer to
Chapter 14 Plant Genetic Engineering Drugs Section 1 Plant Genetic Engineering
1. Construction of Plant Expression Vector
Second, plant transformation.
Section 2 Plant Genetic Engineering Drugs
First, plant genetic engineering recombinant protein drugs
Second, plant secondary metabolic engineering
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refer to
Chapter 15 Overview of Molecular Targeted Drugs Section 1
Section II Molecular Targeting of Cell Signal Transduction Pathway
medical science
I. Ras/MAPK channel inhibitors
Second, protein kinase C inhibitors
Third, cyclooxygenase? 2 selective inhibitor
Fourth, telomerase inhibitors.
Five, Fachini transferase inhibitors
Section 3 Proto-oncogene and Tumor Suppressor Gene
Molecular targeted drugs
First, the characteristics of proto-oncogene expression
Second, the structural changes and forms of proto-oncogenes
Expression activation
Section IV Cytokine Receptor Molecular Targeted Drugs
Section V Molecular Targeted Drugs for Anti-angiogenesis
Section 6 Miniaturized Antibody Targeted Drugs
Section 7 Suicide gene
refer to
Chapter 16 fusion protein drug Section 1 recombinant human tumor necrosis factor receptor-
Fc fusion protein
First, tumor necrosis factor
Section II Recombinant Anti-tumor Fusion Protein
I. Fusion protein and tumor
Second, the fusion protein and the evolution of malignant tumor
Third, the study of fusion protein in tumor treatment and prevention.
Apply to.
Recombinant human serum albumin? interferon
First, the production and structural characteristics of interferon
Second, the therapeutic principle of interferon
Three. Interferon products derived from the market
Section 4 Recombinant Human Fibroblast Growth Factor
I. Structure and function of αFGF and FGFR
Second, the biological function
Third, the current situation of αFGF gene engineering expression
Fourth, the problems that should be paid attention to in clinical application of αFGF.
Section 5 Recombinant human granulocyte macrophage
Colony-stimulating factor
I. human granulocyte macrophage colony stimulating factor
Molecular biological characteristics of
Two. Human granulocyte macrophage colony stimulating factor
Clinical application of.
Three. Human granulocyte macrophage colony stimulating factor
Study on transgenic expression
Section VI Interleukin
I. Types and structure
Second, biological activity.
Third, the function of IL
Think about a problem
refer to
............................................................................................................................................................................
I. Insulin molecules
Two. Insulin receptor and signal transduction
3. Using recombinant DNA technology to prepare insulin products.
Fourth, treat diabetes with insulin synthetic cells.
Human growth hormone in the second quarter
Growth hormone releasing factor and inhibitory factor
Second, the physiological role of growth hormone receptor and growth hormone
Therapeutic effect of growth hormone
Third, recombinant Hugh and pituitary dwarfism
Fourth, Hugh's metabolism.
Growth hormone, lactation and ovulation
Section III Gonadotropin
I. recombinant gonadotropin
Second, the application of gonadotropin in veterinary medicine
Third, gonadotropin releasing hormone.
Section 4 Other recombinant approved for clinical use
hormone
I. recombinant human parathyroid hormone
Second, recombinant calcitonin
Three. Recombinant thyrotropin
Think about a problem
refer to
Chapter 18 Blood products and therapeutic enzymes Section 1 Blood substitutes
First, dextran
Second, albumin.
Third, gelatin protein.
Fourth, oxygen-carrying blood substitutes.
Coagulation factors and hemophilia in the second quarter
I. Coagulation factor VIII
Two. Coagulation factors ⅸ and ⅶ A
Section 3 Therapeutic enzymes
First, antithrombin
Second, thrombolytic agents.
Third, superoxide dismutase.
Four, other therapeutic enzymes
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refer to
Chapter 19 vaccine technology and molecular diagnostic technology Section 1 vaccine technology
I. Preparation of traditional vaccines
Second, genetic engineering vaccine technology and DNA vaccine
Three. Aids vaccine
Fourth, tumor vaccine.
Five, adjuvant technology and mode of action
Section 2 Molecular Diagnostic Techniques
First, the concept and principle of molecular diagnosis
Second, the common technical methods of molecular diagnosis
Section III Clinical Application of Molecular Diagnosis
I. molecular diagnosis and genetic diseases
Second, molecular diagnosis and infectious diseases
Thirdly, molecular diagnosis and tumor.
Fourthly, molecular diagnosis and individualized treatment.
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