High-throughput sequencing platform (high-throughput_genome_sequence_database) Since 454 Life Sciences (formally acquired by Roche in 2007) launched the 454 FLX pyrosequencing platform in 2005, Applied BioSystem (ABI), which has launched the 3730xl DNA sequencer (3730xl DNA Analyzer), has always occupied the largest share of the sequencing market. Its leading position has begun to shake, because their flagship product capillary array electrophoresis sequencer series (series capillary) array electrophoresis sequencing machines) has encountered two strong competitors, one is Roche's 454 sequencer (Roch GS FLX sequencer), and the other is the Solexa genome analysis platform (Genome) launched by the American Illumina company in 2006. Analyzer platform), for this reason, in 2007 ABI launched its independently developed SOLiD sequencer (ABI SOLiD sequencer). These three sequencing platforms are representatives of current high-throughput sequencing platforms.
In recent years, high-throughput sequencing technology has evolved from gel plate-based methods (e.g., "S Radiolabeled Sequencing Manual, ABl 373, ABIPRISM 377, Li-corIR2 DNA Sequencer ) to capillary sequencer (such as ABI Prism 3100, 3700 and 3730xl DNA sequencers, Megabace1000 and Megabace 5000) sequencing, there have been reviews published on methods and equipment for preparing DNA and sequencing (Meldrum, 2000a, b). The shift to capillary sequencers has solved the pathway tracing issues inherent in gel plate technology, and automation of capillary sequencers has significantly increased throughput while providing longer sequenceable lengths. These advances have significantly increased the number of human pathogens produced in recent years. Genomic data.
TIGR's current cutting-edge sequencing equipment includes 25 ABIPrism 3700 DNA analyzers, 24 AppliedBiosystems 3730xl DNA analyzers, and 6 ABIPrism 3100 DNA analyzers. Approximately 50 projects are underway at the same time. More than 3,400,000 sequencing reactions were performed annually, and 6,000,000 reactions were completed by the end of 2002. TIGR's current sequencing capacity is 14,000,000 sequencing processes per year.