The application number is cn20110228564.7, and the application date is 2011.08.10.
Release (announcement)No. CN 10227424 1A Release (announcement) Date 2011.12.14.
Right of validity of current legal status authorization
Classification number a 61k 35/54 (2006.438+0) I; a 6 1k 38/ 17(2006.0 1)I； a 6 1p 37/04(2006.0 1)I； A6 1P39/06
Applicant (patent right) Zhou Wei.
Address 4 1 1228No. Haiji Road, Huarong Industrial Park, Yueyang City, Hunan Province 1
Inventor (Designer) Zhou Wei
right of priority
abstract
The invention provides a method for extracting sheep embryo extract and a pharmaceutical composition containing the extract, wherein the method for extracting sheep embryo extract comprises the following steps: mincing sheep embryo, freezing, dissolving, grinding and centrifuging, and then filtering supernatant obtained in the centrifugation step to obtain sheep embryo extract, and further comprising the step of soaking sheep embryo fragments obtained in the mincing step in water between the mincing step and the freezing step; The centrifugation temperature in the centrifugation step is controlled within the range of-15 ~- 10℃; Supernatant filtration means that the supernatants pass through the ultrafiltration membrane with molecular weight of 6.5438+million and the ultrafiltration membrane with molecular weight of 6.5438+million in turn. The method for extracting bioactive substances from sheep embryos provided by the invention centrifugally extracts supernatant at-65438+/-00 DEG C, so that the bioactivity of the extract can be effectively maintained. After that, ultrafiltration membranes with molecular weights of 654.38+ and 654.38+ were used in turn for filtration, so that protein, which is easy to be obese, was removed, and bioactive substances were retained, so that the extract only had cosmetic effect and did not gain weight.