What is pcr fluorescent probe method?

Quantitative real-time PCR (qPCR) is a molecular biology technique, which is used to amplify and simultaneously detect or quantify target DNA molecules. The plan follows the general principles of PCR.

In the process of PCR reaction, with the increase of the number of cycles, the accumulation of PCR products leads to the enhancement of fluorescence signal. Therefore, by monitoring the fluorescence intensity, the progress of PCR reaction can be detected "in real time". SYBR Green qPCR and T aqMan probe qPCR are commonly used in scientific research.

Fluorescence PCR is an analytical instrument applied in the field of biology, which was put into use on June 5438+1October 3, 2003.

High sensitivity: single copy gene can be detected; Wide dynamic range: 8 copies of 10e0- 10e8 can be detected; High repeatability: CV