According to the different development history, influence, sequencing principle and technology, there are mainly the following kinds: massive parallel signature sequencing, MPSS, Polony sequencing, 454 pyrophosphate sequencing, Illumina (Solexa) sequencing, ABI solid-state sequencing, ionic semiconductor sequencing, DNA nanosphere sequencing and so on.
High-throughput sequencing technology is a revolutionary change to traditional sequencing, which can sequence hundreds of thousands to millions of DNA molecules at a time, so it is called the next generation sequencing in some literatures, which shows its epoch-making change. At the same time, high-throughput sequencing makes it possible to analyze the transcriptome and genome of a species in detail, so it is also called deep sequencing.
Since 454 Life Sciences Company (formally acquired by Roche in 2007) launched 454 FLX pyrophosphate sequencing platform in 2005, the leading position of applied biological system (ABI), which once launched 3730xl DNA analyzer, has been shaken because their flagship product series, capillary array electrophoresis sequencer, met two powerful competitors, and this instrument has always occupied the largest share in the sequencing market. One is Roche's 454 sequencer. The other is Solexa genome analyzer platform launched by Illumina Company in 2006. To this end, ABI Company launched its own solid-state sequencer in 2007. These three sequencing platforms are the representatives of high-throughput sequencing platform at present.