Unless they are of the same gender, they can all mate together.
This article constructed an iCre (improved Cre, optimized Cre recombinase) BAC vector expressing AVP, VIP, and PROK2 neuropeptides through homologous recombination. Through pronuclear injection of fertilized eggs, transgenic mice expressing iCre recombinase in SCN-specific neurons were obtained, which are AVP-iCre, VIP-iCre, and PROK2-iCre. Then the genotype and copy number of the transgenic mice were identified, and the positive mice were mated with Rosa26-mT/mG reporter mice, and the resulting double-positive mice were subjected to SCN sectioning.
The tissue specificity and activity of CRE expressed in these iCre transgenic mice were further determined by observing the distribution of green fluorescent protein and the distribution of SCN-specific neurons. At the same time, the biological rhythms of these transgenic mice were measured in an environment without external light and dark cycles to rule out that their biological rhythms were damaged. The results showed that there was no difference in the biological rhythms of iCre transgenic mice and wild-type mice. AVP-iCre , VIP-iCre and PROK2-iCre mice can be used to study biological rhythms. Therefore, this article successfully constructed three iCre transgenic tool mice.