Q 1, autologous hair follicle regeneration and implantation, sounds a bit novel, can you elaborate on the technical principle?
A: There is a problem that we must be clear about: all hair loss is related to whether hair follicle stem cells work normally. So what are hair follicle stem cells? In the 20th century, Cotsarelis, Taylor and others found that hair follicle stem cells, like stem cells, have strong proliferation ability and multi-directional differentiation potential. It is the primitive cell in hair follicle and the basic unit of hair, and hair comes from the growth and differentiation of cells in hair follicle. A group of hair follicle stem cells live in each hair follicle, providing new cells for growing hair follicles. Hair follicle stem cells are easier to obtain than stem cells from other organs. Hair follicle stem cells are contained in the hair we pull out. If we master the technique of hair follicle stem cell culture in vitro, we can carry out a large number of cultures.
As the saying goes, know yourself and know yourself. We have been doing research on stem cells, and my preference is hair follicle stem cells. My research team and I have also invested in the field of hair regeneration for more than ten years, and made breakthroughs in the purification and culture of hair follicle stem cells, autologous hair follicle regeneration and implantation, and hair follicle preparation. From animals to human body, it can promote hair growth after repeated clinical verification. By extracting a small amount of healthy hair follicles from the occipital region (this is the best range we have obtained through countless experiments, which belongs to the standard amount, balancing the final product and the cell culture time to reach the most appropriate standard), the cells responsible for hair growth are separated and sent to the laboratory for culture and expansion, and hundreds of millions of hair follicle stem cells and hair papilla cells with the function of promoting hair follicle regeneration and repair are cultivated in about 2-4 weeks to form hair follicle primordium for regeneration and implantation. This is equivalent to replanting the long-haired seeds to the hair loss part through our self-developed planting gun to achieve the purpose of hair regeneration.
Q2。 Can you elaborate on the scientific research process? What's the difficulty?
A: Scientific research has always been very long and boring. First of all, we should have good ideas, learn from previous scientific research experience and understand related trends. The understanding of hair follicle stem cells is deepening at home and abroad. You must be familiar with a large number of papers in related fields, have laboratories and sophisticated cell equipment, and members of the research team should have a deep understanding of stem cells, especially hair follicle stem cells. Only by combining these factors can we promote the transformation of scientific research results. Why did you start this research? Because I found that the traditional hair transplant encountered a bottleneck and could not solve the problem of patients with large-scale hair loss. Based on this problem, we began to study hair follicle stem cells, and it has been more than ten years now, and we have repeatedly done relevant experiments and demonstrations. The preclinical study of animal model on 20 15 achieved ideal results, and the first phase of clinical trial was started on 20 19, which achieved good results.
During this period, the most difficult thing is the separation and purification of hair follicle stem cells. Through some specific markers expressed by hair follicle stem cells, we separated the target living cells from heterogeneous cell groups by flow cytometry, and separated CD34+ hair follicle stem cells by flow cytometry. The obtained hair follicle stem cells have high purity and little chance of pollution. The isolated hair follicle stem cells should be put into a culture container with suitable culture medium and cultured in a 5% CO2 incubator with saturated humidity. We should regularly observe the expansion of hair follicles and the activity of cells, just like raising children. Our team members often say, look what our children have grown up to be.
Q3。 What is our current clinical situation and can it be widely used? How safe is it?
A: The previous research team did a lot of research, and according to the research and development process, the animal model of androgen alopecia was used for preclinical research, and the ideal experimental results were obtained. Then, we conducted a small-scale clinical study and achieved good results. For example, we successfully planted more than 20 cases last year, and 100% grew new hair. The earliest case of/kloc-0 was 9 months, and the hair coverage rate reached over 80%. Customers who found us through online platform tracing activities and implemented autologous hair follicle regeneration also grew well in 3 months.
We strictly follow the steps of scientific research, from preclinical research to clinical research of animal models, and have achieved good results. Moreover, our technology is to extract a small amount of healthy hair follicles from customers and send them to a self-built laboratory for in vitro culture. Medically speaking, this is autologous, and there is no rejection. Moreover, hair follicle stem cells are pluripotent stem cells, which will not develop into other tissues and will not become cancerous. This is very important. Our researchers, all with doctoral degrees, have conducted in-depth research in the field of stem cells, especially hair follicle stem cells for many years, and conducted repeated experiments in the laboratory to ensure the health and activity of the cultured hair follicle primordia for planting, and then adjusted the appropriate dosage according to the customer's own situation, which is completely from personal tailor. We also have our own laboratory. After the hair follicle is extracted, it will be sent to the laboratory and handed over to the researchers. They will be differentiated and cultured into hair follicle primordia through advanced cell equipment, which ensures the activity to the greatest extent.
Q4。 What are the advantages compared with traditional hair transplantation?
A: The most fundamental difference is that the FUE technology has solved the problem of hair loss by robbing Peter to pay Paul, but it can't solve the problem of large-scale hair loss and insufficient resources of the back pillow. Our technology has broken through the limitation that traditional surgical treatment can't solve the "congenital deficiency" of autologous hair, especially for patients with severe alopecia above grade 6, only a few healthy hair follicles need to be cultured and proliferated in vitro, and hundreds of millions of hair follicle primordia can be cultivated for replantation, thus avoiding the embarrassment of insufficient resources.
The realistic problem faced by FUE hair transplantation is that the survival rate is not ideal, which is mainly affected by many factors during the extraction process, and the survival rate can not reach 100%. Our technology is not limited by resources. Autologous hair follicles are sent to GMP laboratory for culture, and expand infinitely, which is equivalent to a newborn. Each one is fresh and full of vitality, and then planted in the hair loss area. The effect can be imagined. Moreover, we will preserve the cultured second generation hair follicle stem cells for free, because everyone's aesthetics will be different at different stages. If you want to plant it again in the future, you can directly use the young cell species preserved in our laboratory without extraction. Generally speaking, our technology is not affected by hair loss area and our own hair resources, and has no rejection, side effects and adverse reactions, minimally invasive and painless, short operation time, rapid recovery and high coverage.
Q5。 How long can the hair last after hair transplantation? Will it fall off in the future?
A: Every hair does not grow endlessly, but has a growth cycle. Does the growth cycle of normal hair include the growth period? There are three stages: the fading period and the rest period. The growth period lasts 2-6 years, which is the key stage of hair formation. Endocrine disorders, hormone secretion, stress, medication, burns, trauma, diseases and malnutrition may all stop the normal growth of hair; In the regression period, less than 1% of hair follicle stem cells stop working, which generally lasts about 1-2 weeks. During this period, the hair roots contract and the hair gradually moves from the hair roots to the upper part of the scalp; In the quiescent stage, about 10% of stem cells are quiescent for about 5-6 weeks, during which the hair will automatically fall off. After enough rest, stem cells will return to the growth stage and then start a new cycle. ?
Healthy adults have about 654.38+ million hairs, most of which are in the growing period. Under normal circumstances, it is normal for healthy adults to naturally lose about 50- 100 hairs every day. As long as we take good care of it, our hair will grow normally. As long as the hair follicle is healthy and well maintained after regeneration and implantation, it can grow normally! Because we extract the long-lived hair follicles in the occipital region as the seeds for planting, in principle, the long-lived hair follicles in the occipital region will not fall off at the back and front, but with the growth of age, the body's functions will degenerate and naturally age, and even the long-lived hair follicles in the occipital region will fall off.
Q6。 How to treat the voice of doubt on the internet?
A: Scientific research is a long process, and it is a long process for scientific research results to be accepted by the public. It is normal to have doubts, which shows that everyone is paying attention to our scientific research results at present.
Second, there are many non-scientific research institutions in the market under the guise of scientific research, which disturbs the market order, makes many people deceived, and also complicates the normal work of our real scientific research institutions;
Third, our technology is not omnipotent, and there is a lot of room for optimization. At present, this technology is mainly aimed at androgenetic alopecia and alopecia areata, but it is not suitable for pathological alopecia (caused by skin diseases and scars), which we can't solve at present and is also the theme we will overcome in the next step. Another thing we have been considering is how to reduce the cost and make it acceptable to more people with hair loss without changing the technical effect, which we need to constantly optimize.