Patent application date:1994.10.19.
Name: Method for preparing 5'- mononucleotide by continuous enzymolysis of ribonucleic acid.
AnnouncementNo.: cn1121112.
Date of publicity (announcement): 1996.04.24
Category: chemistry; metallurgy
Application (patent right): Dalian Institute of Chemical Physics, Chinese Academy of Sciences
Address: Zhongshan Road116012+061,Dalian, Liaoning.
Inventor (Designer): Shao Jingping; Guo Chunxiang; Liu Yuxin; Guo hetian
Patent Agency: Shenyang Patent Office of China Academy of Sciences.
Agent: Zhang Chen.
Abstract: A method for preparing 5'- nucleotide by continuous enzymolysis of ribonucleic acid is characterized in that nuclease is firstly injected into an ultrafiltration membrane reactor; Continuously pumping RNA buffer solution, keeping the temperature at 60 ~ 70℃ and the pumping speed at 0.5 ~ 65438 0.5 ml/min; Collecting the permeate, separating, concentrating and crystallizing to obtain the 5'- nucleotide product. The invention can be used for continuous production, convenient operation, low cost and high hydrolysis rate.
Sovereign projects:
A method for preparing 5'- nucleotide by continuous enzymatic hydrolysis of ribonucleic acid is characterized in that an ultrafiltration membrane reactor is used in the hydrolysis process, and the process flow is as follows:-injecting nuclease P 1 into the reaction chamber of the membrane reactor (5), wherein the concentration of nuclease P 1 00-65430. -keeping the temperature of the membrane reactor (5) at 60-70℃, continuously pumping RNA ester acid buffer with the concentration of 1- 10%, the pH value of the buffer is 5.0-6.0, and the buffer also contains 0.01-0/mm. Collecting the permeate, separating, concentrating and crystallizing to finally obtain 5'- cytidine, 5'- guanylic acid, 5'- adenosine and 5'- uridylic acid.