What should I do to observe the slices of roots and leaves of plants?

Usually, these plant materials are sliced by freehand slicing method, and then made into temporary slices by temporary specimen method, and observed under a microscope.

1. Clean the slide:

Wash the slide and cover glass with clean water, and then dry them with gauze. When wiping the glass slide, the left index finger and thumb hold both sides of the glass slide, and the right index finger and thumb wrap gauze. At the same time, wipe both sides of the slide evenly and forcefully.

Note: Cover glass is fragile, so be careful when wiping, handle it gently and apply force evenly.

2. Place materials:

Put the glass slide flat on the table, add a drop of distilled water or diluted glycerin in the center, then take a small amount of plant material with tweezers or suck it with a straw, put it into the water drop or glycerin, and then carefully flatten or spread the material with tweezers and dissecting needles to avoid folding or drying.

3. Cover:

Pinch the two corners of the cover glass with your thumb and forefinger or clamp one end with tweezers, so that one side of the cover glass first contacts the edge of the water drop on the glass slide, and then slowly put down the other side. When the clamped side of the cover glass will be close to the glass slide, you can let go or pull out the tweezers to cover it naturally and gently, so that the air under the cover glass can be squeezed out and bubbles can be avoided. ?

Note: The amount of droplets in the center of the slide should be moderate, so that it just fills under the cover glass. The top of the cover glass and other areas of the slide must be kept dry. If the amount of droplets is not enough, bubbles will easily occur. A small drop of water can be carefully dropped from the side of the cover glass with a straw to make it contact with the water phase under the cover glass. If too much water overflows the cover glass, the cover glass will float or overflow from under the cover glass. At this time, you can suck it with absorbent paper.

4. Dyeing:

If dyeing is needed, directly after the temporary water-filled film is made, drop a drop of dye solution on one side of the cover glass, absorb the water on the other side of the cover glass with absorbent paper, introduce the dye solution, and let it stand for a while to make the substances under the cover glass evenly colored.

5. Microscopic examination.