Zhanqin Zhao, male, born in 198, doctor, professor, doctoral supervisor, academic leader of the veterinary biological products engineering team of Henan University of Science and Technology. In 28, he graduated from Huazhong Agricultural University, majoring in preventive veterinary medicine. In the same year, he went to the College of Animal Science and Technology of Henan University of Science and Technology for teaching and research. From May 215 to May 216, he worked as a visiting scholar in the College of Veterinary Medicine of the University of Georgia. Long-term commitment to scientific research, teaching and production, and commitment to scientific research, personnel training and technical services. The research direction is the research and development of livestock infectious diseases and their vaccines. In terms of basic research, focusing on human and animal suffering from bacterial infectious diseases, we mainly engage in the research on the cross-species transmission mechanism of "superbacteria" such as multi-drug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and their host susceptibility correlation. In the application research, the research focuses on the vaccines of important livestock and poultry bacterial infectious diseases, and the research on the biology, technology, development and creation of veterinary biological products is in-depth.
Chinese name: zhanqinzhao
mbth: zhanqinzhao
Nationality: China
Nationality: Han
Birthplace: Lankao, Kaifeng, Henan
Date of birth: October, 198
Occupation: teacher
Graduation institution: Ph.D. from Huazhong Agricultural University
Belief: Party member
Major achievements: research and development of animal infectious diseases and vaccines
Representative works: research and development of vaccines for major bacterial infectious diseases in pigs
Profile
Zhanqin Zhao, male, born in 198, is a doctor, professor, doctoral supervisor and academic leader of the veterinary biological products engineering team of Henan University of Science and Technology. In 22, he graduated from the College of Veterinary Medicine Engineering of Henan Agricultural University, majoring in veterinary (health inspection); In the same year, he went to the State Key Laboratory of Agricultural Microbiology of Huazhong Agricultural University to study for a doctorate in preventive veterinary medicine (master's degree and doctoral degree). His tutors were Academician Chen Huanchun and Professor Wu Bin, famous experts in livestock infectious diseases. During his doctoral studies, he was mainly engaged in the separation and identification, serotyping and genotyping of streptococcus suis, Escherichia coli, Bordetella bronchiseptica and Haemophilus parasuis. During this period, he won the "Excellent Postgraduate of the State Key Laboratory of Agricultural Microbiology" award. The doctoral dissertation "Study on the Recombinant Salmonella Genetic Engineering Vaccine of Bordetella bronchiseptica" won the Excellent Doctoral Dissertation Award in Hubei Province.
Since p>28, he has been engaged in teaching and scientific research in the College of Animal Science and Technology of Henan University of Science and Technology, and from May 215 to May 216, he worked as a public visiting scholar in the College of Veterinary Medicine of the University of Georgia. Long-term commitment to scientific research, teaching and production, and commitment to scientific research, personnel training and technical services. The research direction is the research and development of livestock infectious diseases and their vaccines. In terms of basic research, focusing on human and animal suffering from bacterial infectious diseases, we mainly engage in the research on the cross-species transmission mechanism of "superbacteria" such as multi-drug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and their host susceptibility correlation. In the application research, the research focuses on the vaccines of important livestock and poultry bacterial infectious diseases, and the research on the biology, technology, development and creation of veterinary biological products is in-depth. A complete germ plasm resource bank of pathogenic bacteria of livestock and poultry has been established, which contains more than 5,6 strains of main animal bacterial pathogens, such as Haemophilus parasuis, Streptococcus, Bordetella bronchiseptica, Pasteurella multocida and erysipelas erythema, and 32 vaccine strains that meet the epidemic characteristics in China have been screened. More than 7 protective antigens of important pathogenic bacteria were identified by modern molecular biology technology, and combined vaccines and subunit vaccines of five bacterial infectious diseases were developed and transformed. He has presided over 16 scientific research projects such as the National Natural Science Foundation and major horizontal projects of school-enterprise cooperation, and won 18 prizes such as the second prize of scientific and technological progress in Henan Province. He has published 52 research papers in domestic and foreign journals such as InfectionandImmunity, Vaccine, China Agricultural Sciences and Journal of Microbiology, including 21 SCI papers and 2 applications for the National New Veterinary Drug Registration Certificate.
The main research experience
1. From July 23 to June 25, I was mainly engaged in the isolation and identification of Escherichia coli from swine edema disease, and the research on its inactivated vaccine and diagnostic methods. The research stage of the vaccine laboratory was completed, including the screening of Escherichia coli vaccine strains for edema disease, the biological characteristics of the strains, the preparation of inactivated vaccine, the efficacy test of virus attack protection in mice and the efficacy test of virus attack protection in pigs. Guide the completion of the late clinical trial of the inactivated vaccine.
from June 25 to April 26, I participated in the diagnosis and tackling key problems of sudden human swine streptococcosis in Sichuan in 25: I was the main executive appraiser of "swine streptococcosis" in Sichuan province in 25. The molecular biological characteristics of Sichuan virulent strains SC-1, SC-2, SC-3 and other local virulent strains were studied. It laid a foundation for the development of swine streptococcosis vaccine. At the same time, a clinical practical PCR rapid detection kit for Streptococcus suis type 2 was developed.
from may 26 to August 27, the epidemiology of Bordetella bronchiseptica, the main pathogen of atrophic rhinitis in pigs, and the development of genetic engineering vaccine. Including the isolation, identification and molecular biology of Bordetella bronchiseptica, the development of salmonella vector, and the development of Bordetella recombinant salmonella genetic engineering vaccine. The constructed genetic engineering recombination vaccines has been tested in environmental release. At the same time, it obtained one national invention patent.
4. since 22, a large number of veterinary clinical diagnosis and comprehensive prevention and control have been carried out in pig farms all over the country. Especially in large-scale pig farms in Hubei, Henan and other provinces for long-term clinical diagnosis and disease epidemic investigation. A large number of first-hand epidemiological data of pigs were obtained, and a large number of epidemic strains were isolated, including streptococcus, Haemophilus parasuis, Pasteurella multocida, Bordetella bronchiseptica and so on.
5. Since 28, he has been engaged in teaching and scientific research in the College of Animal Science and Technology of Henan University of Science and Technology. His research direction is the research and development of livestock infectious diseases and their vaccines, focusing on the vaccines of important livestock bacterial infectious diseases, and making in-depth research on the biology, technology, development and creation of veterinary biological products. A complete germplasm resource bank of pathogenic bacteria of livestock and poultry was established, with more than 5,6 strains of main bacterial pathogens of animals preserved, and 32 vaccine strains conforming to the epidemic characteristics of China were screened. More than 7 protective antigens of important pathogenic bacteria were identified by modern molecular biology technology, and combined vaccines and subunit vaccines of five bacterial infectious diseases were developed and transformed.
Research Foundation and Technology Platform
The main research direction is the research and development of livestock infectious diseases and their vaccines. In terms of basic research, focusing on human and animal suffering from bacterial infectious diseases, we mainly engage in the research on the cross-species transmission mechanism of "superbacteria" such as multi-drug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and their host susceptibility correlation. In the application research, the research focuses on the vaccines of important livestock and poultry bacterial infectious diseases, and the research on the biology, technology, development and creation of veterinary biological products is in-depth. At present, a large number of research materials and technical means have been accumulated, and the following main research foundations and technical platforms have been laid.
A complete germ plasm resource bank of pathogenic bacteria of livestock and poultry has been established, which contains more than 5,6 strains of main bacterial pathogens of animals, such as Haemophilus parasuis, Streptococcus, Bordetella bronchiseptica, Pasteurella, Erythrina erysipelas and so on, and 32 vaccine strains that meet the epidemic characteristics in China have been screened. More than 7 protective antigens of important pathogenic bacteria were identified by modern molecular biology technology, and combined vaccines and subunit vaccines of five bacterial infectious diseases were developed and transformed. He has presided over 16 scientific research projects such as the National Natural Science Foundation and major horizontal projects of school-enterprise cooperation, and won 18 prizes such as the second prize of scientific and technological progress in Henan Province. He has published 52 research papers in domestic and foreign journals such as InfectionandImmunity, Vaccine, China Agricultural Sciences and Journal of Microbiology, including 21 SCI papers and 2 applications for the National New Veterinary Drug Registration Certificate.
The "Open Laboratory of Key Discipline of Environment and Animal Products Safety in Colleges and Universities of Henan Province" is an excellent laboratory for "double-base" evaluation in Henan Province. The school has animal science and technology college, affiliated animal hospital, medical college and affiliated "National Top 1 Hospitals", which is suitable for the development of scientific research projects such as animal infectious diseases and diseases of humans and animals. ABIVeriti PCR instrument, BIO-RADGelDoc gel imaging system, BIO-RADProteani12 two-dimensional electrophoresis system, Alpha1-2LD vacuum freeze dryer, Sigma3-18KS high-speed freeze centrifuge, multifunctional bacteria-cell converter, ultrasonic cell breaker, PKF bacterial endotoxin detector, BIO-RAD protein two-dimensional electrophoresis instrument, Thermo ultra-low temperature refrigerator, ultra-pure water system, ultraviolet spectrophotometer, automatic enzyme label instrument, Antai SW-CJ-2FD clean bench, Bio-Rad biosafety cabinet and other equipment.
The laboratory open platform of College of Animal Science and Technology of Henan University of Science and Technology has BIO-RADCFX96Touch fluorescence quantitative PCR instrument, Axioboservera 1 inverted fluorescence microscope of Zeiss company, FACSVerse flow cytometer of BD company, OptimaL-8XP ultra-high speed freezing centrifuge of Beckman company, AKTAPure protein purification system of GE company, AllianceE2695 high performance liquid chromatograph of WATERS company, Clarus69 gas chromatograph of PerkinElmer company, PinAAcle9H atomic absorption spectrometer of PerkinElmer company, Avio2 inductively coupled plasma emission spectrometer of PerkinElmer company, Large-scale instruments and equipment such as PerkinElmer Company and Thermo Company's Nanodrop2C ultramicro nucleic acid protein concentration analyzer; There are also experimental animal rooms, animal hospitals, experimental pastures, sterile cell rooms and other facilities.
The open platform of the dynamic laboratory of Henan University of Science and Technology has large-scale instruments and equipment such as HitachiS48 scanning electron microscope and HitachiHT77 transmission electron microscope.
Main scientific research projects undertaken
1. National Natural Science Foundation project, Isolation and identification of NDM-1-producing bacteria from pigs and study on the possibility of human-pig interaction (313216), 214-216, 25, yuan
2. National Natural Science Foundation project, Escherichia coli producing β -lactamase -1 from New Delhi from pigs. 52, yuan
3. Major horizontal project of school-enterprise cooperation, research and development of trivalent inactivated vaccine against Haemophilus parasuis (H216922), 216-2195.2 million yuan
4. Major horizontal project of school-enterprise cooperation, research and development of combined inactivated vaccine against Streptococcus suis and Haemophilus parasuis (H21786). Research and development of pentavalent inactivated vaccine against Haemophilus parasuis (H21917), 218-222,48, yuan
6. Horizontal project of school-enterprise cooperation, development of video system for diagnosis and treatment of veterinary infectious diseases (H218125-2), 219.1-22.12,32, yuan < P >.
8. Fund project for Young Academic Leaders of Henan University of Science and Technology, Study on Subunit Vaccine of Sheep Infectious Pleuropneumonia (215XSD28), main research papers published in 215-217, RMB 3,
.