Several essential elements for a successful tissue culture experiment
1. Experimental environment
A professional plant tissue culture laboratory is a key factor for the success of plant tissue culture one. The entire process of plant tissue culture must be carried out in a sterile environment. From the disinfection, pruning and inoculation of explants to the later cultivation of sterile seedlings, a sterile experimental environment is indispensable. A professional and clean laboratory The plant tissue culture laboratory is an indispensable condition for the success of plant tissue culture experiments. It can greatly reduce the probability of contamination during tissue culture operations and sterile seedling cultivation.
2. Experimental instruments, equipment and consumables
1. Ultra-clean workbench: It is a highly versatile air purification equipment that provides a local high-cleanliness working environment and is a plant tissue The core instrument in culture can provide a cleanliness level of up to 100 levels. Without the sterile environment provided by a clean bench, the success rate of plant tissue culture is almost zero. In a conventional laboratory environment, it is impossible to perform plant tissue culture operations. Even if the experimental operations are completed, the sterile seedlings finally cultured will be contaminated by external microorganisms. Chuanbulan ultra-clean workbench is equipped with an anti-ultraviolet damage device and is a national patent-protected product. This device can prevent students from being harmed by UV light if they forget to turn off the UV light during experiments due to improper operation. It is an essential "weapon" for successful plant tissue culture.
2. High-pressure steam sterilizer: It is another core instrument for plant tissue culture experiments. During the process of plant tissue culture, the culture medium and inoculation instruments are sterilized to achieve sterility. . In the 2012 Beijing Sichuan Bulan Tissue Culture Competition, some schools did not purchase high-pressure steam sterilizers, but used ordinary household pressure cookers to sterilize culture media. (The sterilization temperature of household pressure cookers did not reach the level required to completely kill bacteria. temperature), the final experimental results showed that the probability of contamination reached more than 70, resulting in the failure to complete this tissue culture competition; and most schools that use high-pressure sterilizers to sterilize culture media combined with ultra-clean workbenches can Effectively control the probability of contamination below 10, and some schools even achieve zero pollution. In short, ultra-clean workbench and high-pressure sterilization pot are the decisive instruments for the success or failure of plant tissue culture, and it is more beneficial to choose professional essential tissue culture instruments. The conduct and success of the experiment.
3. Micro-adjustable pipettes and tips: experimental tools for measuring micro-liquids. They are used to measure small and trace amounts of hormone stock solutions and adjust the hormone concentrations required in various culture media. The application rate of micro-adjustable pipettes is also high in other experiments, and some experiments require the instruments used to ensure sterility, that is, they can be sterilized by high temperature and high pressure and irradiated with ultraviolet rays, which cannot be accomplished with traditional pipettes. Micro-volume adjustable pipettes from Chuanblan are designed and developed with this in mind.
4. Light culture rack: a place for sterile seedling cultivation, which provides the light needed for plant growth and can control the light intensity according to the actual situation. If the inoculated tissue culture bottle is directly placed in a general environment, induction failure will occur due to insufficient light intensity. Therefore, it is best to choose a light culture frame to provide more suitable growth conditions for tissue culture seedlings.
5. Light incubator: A place for sterile seedling cultivation. It can not only provide appropriate light intensity during plant cultivation, but also control the temperature of sterile seedling culture. In addition, the light incubator can also adjust the light cycle according to the growth habits of different plants, and is suitable for carrying out extracurricular exploration activities. For example, short-day chrysanthemum varieties can control chrysanthemum flowering or raise insects and small animals by adjusting the length of light time. The light incubator is more powerful than the light culture rack. Its application is not limited to the scope of plant tissue culture, and its management is more convenient. Even if there is no one in the cold and summer seasons, as long as each parameter is set in advance, No worries.