B-MicroStation- full-automatic rapid microbial identification instrument
MicroStation rapid microbial identification instrument can identify more than 2000 kinds of aerobic bacteria, anaerobic bacteria, yeast, and mold. The system has passed FDA certification and is suitable for laboratories of any size. Because the reader is convenient, it saves labor.
Biolog scientists have developed a special carbon source to identify strains through tests. Adding the test sample to the microplate test card with pre-selected carbon source will produce a unique imprint or "fingerprint". The results of this biochemical reaction can be automatically read and recorded in a few seconds, which can eliminate the subjective deviation caused by visual judgment. The reaction result can also be compared with the database data to obtain the final result.
Rapid identification
MicroStation system will automatically analyze and record the results. The identified strains are displayed on the computer monitor together with their reaction charts, and 10 strains with the most closely related reaction charts and other useful statistical data are listed.
knowledgeware
Intelligent software can automatically compensate color and turbidity and eliminate subjective differences caused by visual judgment. The innovative advanced identification map matching algorithm not only considers the overall reaction imprint, but also considers the best carbon source of species. The relationship between species and genera of microorganisms established based on carbon source application method is output in the form of system tree diagram or cluster diagram.
Basic test scheme
Since 1989, Biolog has provided microbial identification instruments and related products for various laboratories. Biolog's patented microplate is used for biochemical identification and analysis of the metabolism of bacterial strains, which can identify a variety of bacteria and yeasts. The evaluation plan includes the following four basic steps.
I. Identification principle
On the basis of a lot of research, 95 different carbon sources were screened out for each type of microorganisms, fixed in 96-well plates with different redox reaction chromogenic agents, inoculated with bacterial suspension and cultured for a certain period of time. The absorbance and turbidity were measured by the instrument and compared with the standard strain database, and the final identification result was obtained.
Second, the main technical parameters
1. Hardware composition: reader, turbidimeter, electric eight-head liquid feeder, colony magnifying glass and software.
2. Latest software and database versions: software version 4.2, database version 6.0 1.
3. Identification of microbial species and time spent
Microbial species
Recognizable quantity
Time spent on identification
Gram negative aerobic bacteria
524 species
4-24 hours
Gram-positive bacteria aerobic bacteria
34 1 species
4-24 hours
Anaerobic organism
36 1 species
20-24 hours
yeast
267 species
24-72 hours
filamentous fungi
6 19 species
72 hours or more
4. Identify the type and purpose of the board.
Microbial identification species
use
GN2
Used to identify gram-negative aerobic bacteria.
GP2
Used to identify gram-positive aerobic bacteria.
Art. 1 (before vowels and phonemes)
Used for identification of anaerobic bacteria
Yukon territory
Used to identify yeast.
fire fighting
Used for identification of filamentous fungi
ecological
Used for microbial characteristics and community analysis (3 1 carbon source)
MT2
Used for microbial metabolism research (no carbon source)
San Francisco -N2
Used for metabolic study of gram-negative actinomycetes and fungi.
San Francisco -P2
Used for metabolic study of Gram-positive actinomycetes and fungi.
Note: Unless otherwise specified, there are 95 kinds of carbon sources.
5. The coincidence rate of identification results: over 80%.
Third, the main features
1. In principle, based on the utilization rate of carbon sources, there are as many as 95 kinds of reactions used for identification, and the identification results are highly specific, with great separation and huge expansion space.
2. Compared with the traditional endpoint database, the patent progressive database is easier to get correct results and has stronger anti-interference ability.
3. The capacity of 3.BIOLOG microbial identification database is the largest in the world at present. At present, the total number of microorganisms that can be identified is 1973, covering almost common industrial, agricultural and pathogenic microorganisms. More than 600 kinds of filamentous fungi (molds) can be identified for the first time, such as common Penicillium, Aspergillus, Anthracnose, Fusarium, Trichoderma and so on.
4. Intelligent identification software, users can generate user-defined database, system tree diagram or cluster diagram, which is especially suitable for microbial basic and applied research fields.
5. The original algorithm analysis model comprehensively considers the matching degree and separation effect between the analysis results and the standard database, and truly evaluates the quality of the identification results.
6. The classification of appraisal boards is simple, and there are only five kinds of appraisal boards, which does not require high professional level of operators. In addition, four evaluation boards are designed for the research field.
7. The identification process is simple, the pre-analysis of strains is simple, and only some routine work is needed, such as Gram staining of bacteria, oxidase test of Gram-negative bacteria and iron trisaccharide agar test. Other microorganisms do not need to do any pre-analysis. The identification of mold does not require any experience in fungal identification.
8.BIOLOG has a broad user base in China. At present, there are about 58 users covering various fields.
Fourth, the identification step
At the beginning of identification, the pure strain was isolated according to the conventional microbial operation method. The identification steps are as follows:
1, plate expansion culture: use special culture medium of BIOLOG to expand pure culture;
2. Preparation of bacterial suspension: Prepare bacterial suspension with certain turbidity (cell concentration) as required;
3. Inoculation culture: Inoculate the bacterial suspension into a microplate and culture for a certain period of time;
4. Obtain the result: put the cultivated appraisal board into a reader to read, and the software will automatically give the appraisal result;
Application field of verb (abbreviation of verb)
■ Soil microorganisms ■ Food enterprises ■ Environmental protection ■ Marine life/aquatic products ■ Cosmetics.
■ Pharmaceutical ■ Biological Treatment ■ Agricultural Microbes ■ Veterinary Microbes ■ Biological Community Research
■ Plant pathology ■ Biotechnology ■ Biological control ■ Clinical ■ Quality control of industrial products.