Agrobacterium-mediated transformation refers to the transformation technology that uses Agrobacterium to transform recipient cells and insert the target gene into the genome of recipient cells. This method is the most studied, the most clear mechanism, the most widely used and the most mature plant transgenic technology. Direct DNA insertion method refers to the method of inserting foreign target gene without plasmid vector transformation.
Extended data:
Matters needing attention in plant transgenic technology:
Users should pay attention to the research of functional genomics and molecular breeding. Because there is no need for plant tissue culture, benzyl adenine hormone and pH adjustment, inflorescence infiltration method is simple, rapid, efficient and practical. With the further improvement of this method, it will show greater potential in the field of plant genetic engineering and its derivatives.
At the same time, for species that are difficult to be transformed in vitro by tissue culture, if the flowering period is concentrated or the concentration of flies behind the main fly is removed, and the seed quantity is large, this method can be tried for transgenic.
Baidu encyclopedia-transgenic plants
Baidu Encyclopedia-Transgenic Technology