How many domestic enterprises have the technology of cultivating bezoar in vitro?

The "in vitro cultivation of bezoar" successfully developed by Professor Cai Hongjiao from Tongji Medical College of Huazhong University of Science and Technology was included in the "863" program in 22 and won the second prize of the national technological invention in 23, after being awarded the national invention patent in 1993 and the national first-class Chinese medicine certificate in 1997. In September 23, the National Pharmacopoeia Committee organized academicians Wang Yongyan, Professor Gao Xuemin, Professor Zhang Boli, Researcher Zhou Chaofan, Chief Pharmacist Yao Damu and other well-known domestic authoritative experts to make a special trip to Wuhan for on-site inspection and certification, and agreed that: "In vitro culture of bezoar is mature, stable in quality, safe and effective, and can be used equally with natural bezoar." In January, 24, the in-vitro cultured bezoar was officially approved by the State Food and Drug Administration to be used in the same amount with natural bezoar to replace the natural bezoar in Chinese patent medicines, especially in acute and severe treatment drugs.

: "Cultivating Calculus Bovis in vitro" is to use modern bioengineering technology to simulate the principle and biochemical process of gallstone formation in cattle in vitro. After bacterial culture, under the action of various enzymes, the antagonistic components of gallstone formation in bile are removed to make Shi Niu bile, and then through the promotion of zinc acetate, the gallstone core is formed. Due to electrostatic attraction and bridging effect of organic polymer substances, it is deposited to the core layer in a net shape and grows into bovine calcium bilirubinate stone, thus cultivating.

The test results of quality standards approved by the state show that its technical parameters, quality indicators, functions and indications are consistent with those of natural bezoar. After drug testing and double-blind method, more than 1,85 clinical experiments were carried out in 7 hospitals in three provinces. The results showed that the quality of cultured bezoar in vitro completely met the requirements of Chinese Pharmacopoeia, and its efficacy and performance were very close to or even better than natural bezoar, and its main pharmacological components were more stable than natural bezoar, so it was an ideal substitute for natural bezoar.