How to rejuvenate Ganoderma lucidum strains?

In order to maintain the characteristics of excellent strains and prevent the degradation of domesticated Ganoderma lucidum for cultivation. When separating and screening strains, corresponding preservation methods should be adopted to delay the degradation of strains and prolong the utilization rate of excellent strains. In addition, it is required that every separation and propagation will not cause adverse damage to the strain, so that it can maintain excellent strain characteristics and vigorous growth potential. The culture temperature should not be too high or too low, the high temperature should not exceed 28℃, and the low temperature should not be lower than 18℃. The culture medium should be suitable for the growth of Ganoderma lucidum strains, and the water should be slightly stained between the fingers when it is clenched by hand. The age of bacteria used is not more than two months; Prevent mechanical injuries and burns during vaccination; When separating and expanding, surface hyphae should be discarded. Cultivated species for production are prone to degeneration after repeated tube transfer, so it is necessary to take several branches from the mother species and expand them into cultivated species to prevent the cultivated species from declining due to excessive tube transfer. When the existing strains degenerate, measures should be taken to rejuvenate them. In addition to improving the nutritional conditions of the culture medium, the most effective method is to cultivate the fruiting body, that is, to meet the conditions for producing the fruiting body of Ganoderma lucidum, so that it can complete the sexual generation, and take the fruiting body of Ganoderma lucidum for tissue separation. Select strains with strong vitality, high luminosity, fast growth and high yield, and culture them separately to update the degraded strains. Enriched culture medium can also be used for rejuvenation.