Shandong Bioinformatics Engineering Technology Research Center, established with the approval of Shandong Science and Technology Department in February, 20001,is the only scientific research institution specializing in bioinformatics and computational biology in Shandong Province. The director of the research center is a famous mathematician, the founder of China's finite geometry research, one of the earliest mathematicians engaged in coding and cryptography research in China, and Professor Wan Zhexian, an academician of China Academy of Sciences. The director of the Academic Committee is Professor Zhang Chunting, a famous theoretical biologist and academician of China Academy of Sciences. With a total area of more than 500 square meters, this center has laboratories such as computational biology laboratory, bioinformatics laboratory, protein functional analysis laboratory, natural product activity and pharmacology laboratory, and cell culture room. The center has a research team that is in the prime of life, with reasonable collocation and overlapping professional research backgrounds.
At present, the center's main research directions include: computational biology, bioinformatics, computer-aided drug design, protein structure-function analysis, natural product activity and pharmacology. The center has long been committed to the international frontier and hot topics such as the pathogenesis of neurodegenerative diseases and drug research and development, the structure-activity relationship of bioactive molecules, pharmacology and drug resistance mechanism. , and achieved a series of landmark achievements, and gradually formed the research characteristics of "combining the advantages of theoretical research with experimental research to explore frontier topics in the field of biology".
In recent years, our center has published more than 50 papers on1in SCI retrieval, including a series of papers on N. Engl.J.Med (if: 5 1.7) and Trends Biochem. SCI。 (if: 13.0) and Genome Biol. (If: 10). Trend Moore. Medicine. (if: 9.6), embo rep. (if: 7.2), bio essays(if:5.5), free radical biology. Medicine. (If: 5.3), J. Alzheimer's Harmo's disease. (if: 4.2), bone (if: 4.66). The published papers are widely cited by peers at home and abroad, and the highest citation of a single paper exceeds 100 times. The related achievements won the second prize 1 item of the Natural Science Award of the Ministry of Education, two first prizes and two second prizes 1 item of outstanding scientific research achievements of universities in Shandong Province. In recent years, he has presided over more than 863 National Plan, National Natural Science Foundation and Shandong Natural Science Foundation Project 10. In terms of academic exchanges and cooperation, in recent years, the Center has established close cooperative relations with many domestic and foreign scientific research institutions, such as Ohio State University and Milan University in Italy.
At present, the center is one of the key scientific research institutions of Shandong University of Technology and Shandong Province. We will adhere to the principles of giving full play to our advantages, highlighting our characteristics, being brave in innovation and attaching importance to transformation, and strive to build ourselves into an open, mobile and internationally influential academic group.
[2] Laboratory of Evolutionary and Developmental Biology
The laboratory of developmental and evolutionary biology is a research institution approved by the school and one of the research directions of the master's degree in biochemistry and molecular biology. At present, a laboratory with high technical level has been built, which integrates developmental biology, molecular biology, immunology, evolutionary biology and bioinformatics. The laboratory now has ABI real-time PCR instrument in the United States, TECAN ELISA instrument in Sweden, Zeiss advanced microscope in Germany, Beckman ultra-high speed centrifuge, AKTA FPLC protein separation and purification system of Amerson Bioscience Company in the United States, Hitachi F-4500 fluorescence spectrophotometer, Biometra Tgradient 96 gradient PCR instrument, Japanese Eyela frozen protein drying system, Amashanm's ultraviolet spectrophotometer, nucleic acid hybridization system, ultrasonic pulverizer, high-temperature autoclave and high-temperature dryer have laid a foundation for high-level development and evolutionary biology research.
The Laboratory of Developmental and Evolutionary Biology mainly takes amphioxus, planarian and cells cultured in vitro in model animal as research objects, and uses modern biotechnology to study gene expression and function related to development, regeneration and innate immunity, and combines bioinformatics methods to analyze the origin and evolution of animals. Specific content and characteristics:
(1) Basic research: Through the combination of comprehensive experiments and theoretical research methods, the subcellular and genetic differentiation and evolution mechanism of lower eukaryotes (such as planarian) and protozoa (such as amphioxus) are further studied, and the origin and evolution of animal embryo development, nerve regeneration and natural immunity are deeply studied at the levels of nucleic acid, protein and their complex networks, and their functions are explored. It covers the development and evolution, gene expression regulation, protein function and other biological hotspots.
(2) Applied basic research: To study the effects of exogenous substances (such as pollutants and drugs) on the behavior and gene expression of planarian and cultured cells, focusing on the ecotoxicological and pharmacological effects of pollutants and drugs on planarian at molecular and biochemical levels. It provides methodological and scientific basis for rapid ecotoxicological diagnosis and risk assessment of low-concentration long-term exposure and provides theoretical reference for drug screening.
After several years of development, the laboratory of developmental and evolutionary biology has formed a relatively stable research content and research echelon, and formed its own research characteristics. Good research has been accumulated in the functional genes and natural immune factors of amphioxus, and certain scientific research results have been achieved. Secondly, the regulation of nerve regeneration gene and the formation of the axis of turbot were studied, which laid the foundation for the future work.
There are 2 professors, 3 associate professors and 2 lecturers in the laboratory, including 7 doctors. Undertaking the National 863 Plan 1 project, the National 973 Plan 1 sub-project, the National Natural Fund 1 project, the Shandong Provincial Natural Fund and the Ministry of Education 1 key research projects; In the past three years, 38 academic papers have been published, including 7 articles included in SCI/EI 1 article, and the first prize of Shandong Natural Science Award (2007)1article. The laboratory of developmental and evolutionary biology has a strong faculty and a dynamic research team, forming a research direction with obvious advantages and characteristics. We sincerely invite people with lofty ideals and young students at home and abroad to join our team.
Director: Zhao Bosheng
[3] Plant Molecular Biology Laboratory
Our laboratory mainly studies the regulation of reactive oxygen species, hormones, calcium and other signal molecules on plant growth and development, gene expression and secondary metabolism under adverse conditions. Its characteristics are as follows
1. Signal transduction and regulation of plant organ morphogenesis.
Using rice as a model plant, the relationship between the regulation of redox state of cells and the signal transduction of reactive oxygen species, hormones, calcium, nitric oxide and sugar and the formation of plant organs, especially roots, was studied, and the molecular mechanism of regulating organ morphogenesis under adverse conditions was explored. Taking Arabidopsis thaliana and rice as model plants, the physiological functions and toxicity of rare earths were studied, and the relationship between their activities and toxicity was explored, especially the relationship between the exciting effect of rare earths on plants (low promotion and high inhibition) and the signal transduction and gene expression of calcium and reactive oxygen species.
2. Regulation mechanism of secondary metabolism under adverse conditions and its relationship with plant stress resistance. Taking Suaeda salsa and other plants with strong stress resistance as materials, the relationship between calcium signal and lycopene accumulation, synthesis-related gene expression and plant stress-related gene expression under stress conditions was explored. Taking Haematococcus pluvialis as the material, the relationship between the expression regulation of plant hormones, astaxanthin synthesis key enzyme genes and astaxanthin accumulation was studied, and stress physiology was organically combined with secondary metabolism.
3. Using genetic engineering technology to transfer important genes into plants to improve the quality and stress resistance of cash crops and provide theoretical basis for molecular breeding of crops; Through genetic improvement, the metabolism of microalgae is regulated, and the yield of metabolites with economic value such as astaxanthin and fat is increased, which lays the foundation for pigment separation and biodiesel development.
Our laboratory has made innovative research on the regulation of rare earth in plants, the signal transduction of reactive oxygen species, hormones, calcium and organogenesis, and the regulation of pigment metabolism under adverse conditions, and has made important progress, which is rare in China. We have a solid foundation, accumulated rich research experience and established a research platform and experimental system. At present, the research level has certain advantages in similar research at home and abroad.
Our laboratory has a stable research content and a reasonable research team. Professor 1 person, 4 associate professors, including 6 doctors. Some research achievements have been made, including the National Natural Fund Project 1, Shandong Agricultural Improved Varieties Project 1, Shandong Science and Technology Key Project 1 and Zibo Science and Technology Plan Project. The invention patent 1 was approved, and in 2009, it was awarded the National Natural Fund Project 1; From 2005 to 2009, 89 academic papers were published in core and above journals, of which 27 were included in SCI.
Director: Zhao Fengyun
[4] Laboratory of Bioactive Molecules and Functions
The main research contents in this direction are the biological control of plant diseases, biological vaccines and immunology, and the study of microbial secondary metabolic active molecules in environmental treatment and restoration. The specific research contents and characteristics are as follows:
(1) Study on antagonistic activity and disease-resistant mechanism of biocontrol microorganisms: In view of the serious vegetable crop diseases and the serious "3R" caused by chemical pesticide residues, the microbial strains with high biological antagonistic activity were screened by metagenome technology, and the strains were genetically modified by molecular biology technology to construct high-efficiency antagonistic bioengineering strains, and the disease-resistant mechanism of antagonistic strains was further discussed from morphological, physiological, biochemical and molecular perspectives. In this paper, the screening and genetic transformation of biocontrol strains of tomato gray mold were discussed in depth, which was well received by domestic colleagues.
(2) Molecular virology and molecular immunology of pathogenic microorganisms: screening of active antigens of pathogenic microorganisms, development of vaccines and preparation of specific antibodies; Preparation of immunoregulatory active molecules (adjuvants, cytokines); Screening and expression of bioactive polypeptide molecules; Study on the functions of amino acids and their derivatives. Pioneering work was carried out in the field of soluble expression of hepatitis C virus protease in yeast system, which laid a solid foundation for further study on the pathogenesis and prevention and treatment technology of hepatitis C virus.
(3) Microbial environmental biotechnology research: Microbial biotechnology is mainly applied to environmental protection, and research and technology development are carried out in all aspects of environmental protection, such as water pollution control, degradation of toxic and harmful substances, development of clean and renewable energy, waste recycling, environmental monitoring, and restoration of polluted environment. In-depth research in the field of toxicity mechanism and remediation technology of heavy metals to plants, published papers have attracted the attention of colleagues at home and abroad.
A stable research team has been initially formed in this research field, with/kloc-0 professors, 3 associate professors, 3 lecturers and teaching assistants. Among them, there are 3 doctors, 1 doctor and 2 masters. In the past five years, he has presided over and participated in the National Natural Science Foundation, the National Nano Center Cooperation Project, the Shandong Provincial Natural Science Foundation, the Agricultural Improved Seed Project and other projects 10, and published 58 high-level papers in academic journals at home and abroad, 8 of which were included in SCI or EI, and initially carried out the preliminary work in this field, which was recognized by peers at home and abroad. In the future research, the research team will continue to strengthen the investment in this direction, expecting to be in a leading position in the province and in an advanced position in China.
Director: Ma Huiquan