Basic introduction of Chinese scientific names: fluorescent fish alias: golden zebra; Zebrafish Kingdom: Animal kingdom: Lepidoptera distribution area: India (the predecessor of fluorescent fish-golden zebra) brief introduction, luminous reasons, marine re-breeding, species discovery, new product introduction, breeding status, utilization value and breeding solutions. This fluorescent fish is different from the green fluorescent fish published in 200 1. This time, the coral red gene was produced by genetic engineering and gene transfer technology. In addition, TISCO also demonstrated the colorful fluorescent world for the first time. For the first time, all kinds of fluorescent zebrafish No.2 developed one after another are put in a tank, so that the four colors of red, green, purple and yellow can reflect each other under the short-wavelength black and blue light irradiation, which truly shows the colorful fluorescent world! Fluorescent fish Taiganghong 1 The fluorescent gene plasmid of red coral was injected into the animal polar or embryonic cells of fish eggs by microinjection, so that the host showed the genetic characteristics brought by the gene. Lin Xuelian, manager of the R&D Center of Biotechnology in Taigang, said that unlike in the past, this fish was developed from herring, creating the world's first full-body red fluorescent gene fish. The pink fluorescent charm of the whole body can not only generate new energy, but also create an excellent magnetic field. For China children who love red for happiness, it is really the best feng shui fish. At the same time, TISCO technology has been committed to multi-color research and development, hoping to produce more colors. Therefore, it tried to transplant green jellyfish gene and red coral gene into wild zebrafish with deep body color patterns by using different kinds of zebrafish and muscle luminescent activator, and then successfully cultivated fluorescent zebrafish with various colors. Today is the first time to show more colors of TISCO No.2 fluorescent fairy, which makes the fluorescent fish truly colorful. Fish shine because they pursue a mate. The marine culture of fluorescent gene fish not only has ornamental value, but also has the function of marine culture Fang Zuxi, general manager of Dagang Company, said that the development of genetically modified marine organisms will eliminate the need to collect organisms in coral waters, thus protecting the beautiful coral reef waters around the world from being destroyed. In addition, Fang Zuxi also stressed that replacing the direct capture of marine life with the beauty of gene transfer not only protects the marine ecology, but also applies the technology developed by biotechnology to leisure life, which is the most direct and effective feedback action. Species Discovery At present, scientists have found that many fish like red tones, and some fish have real fluorescent colors and vibrant tones on their body surfaces. Nico Michaels of the University of Tubingen, Germany, who is in charge of this research, said that so far, many researchers believe that red is not very popular in the aquatic world, and the red wavelength in sunlight can be absorbed directly on the sea surface. According to the permeability of sunlight wavelength to seawater, it means that objects are more likely to turn red in air, gray in shallow water and black in water below 33 feet. Unlike red, blue-green light can penetrate deeper below the sea surface, which is why the ocean looks blue. However, recent research findings show that fish have a unique way to display red tones. Michaels said: "This latest research will be published in the upcoming issue of BMC Ecology, which shows that red fluorescence is ubiquitous in marine fish." While diving between Mangrove Bay and Red Sea, michaels accidentally discovered a fish with red fluorescence, which is called "highcrest triplefin fish". In this sea area, he deeply analyzed how crimson light penetrated into the water. He put on a diving mirror to make sure that he could only see red light. He found this strange red fluorescent fish unique-it has red spots around its eyes, and the red fluorescence illuminates the whole body or covers the fins. Michaels said, "The most impressive thing is the red fluorescence around the eyes, and its body seems to be completely illuminated." He pointed out that this red fluorescence is probably a secret communication or a signal to attract attention. But the exact reason has not been confirmed. This is because the red fluorescence comes from the fish, not from the filtered light on the water surface. The red fluorescence emitted by this fish can be seen clearly in the depths of the sea, but its true face can only be seen at close range. He told the American life science website, "We think this is the first time that this red fluorescent fish has been found in the ocean, and now it is regarded as an important phenomenon in coral fish." It is reported that many fish show the ability of blue and green fluorescence. In 2005, a jellyfish was confirmed to emit red fluorescence. The phenomenon of emitting fluorescence is usually due to absorbing light of one wavelength and then releasing it at another very close wavelength. This red fluorescent fish absorbs the blue-green wavelength of light and then releases the red wavelength. Michels guessed that red fluorescence is a common phenomenon in corals and other fish. Because of this first discovery, he also found many other fish with different colors of fluorescence in the Mediterranean. In 2005, five kinds of fluorescent gene fish were newly developed, namely: TISCO 1 new diamond strain-green diamond night pearl, red diamond night pearl and golden diamond night pearl; And the new platinum strains of TISCO No.2-Bai Jin Hong Bao and Bai Jin Lv Bao. Current situation of fluorescent fish culture In the laboratory of Beijing Fisheries Research Institute, there are more than 20 goldfish swimming in a fish tank about 1.5 meters long. When the researchers put the waterproof ultraviolet lamp into the water, the goldfish will emit magical fluorescence. According to the personnel of the Institute of Fluorescent Fish Culture, this is the first batch of fluorescent ornamental fish cultured by transgenic technology in the world, with a total number of 105. The research on transgenic fluorescent fish in the Municipal Fisheries Research Institute lasted nearly 1 year. There were 527 goldfish * * obtained by introducing fluorescent genes in the first batch, of which 105 goldfish fluoresced in varying degrees, and some goldfish even fluoresced all over their bodies. The researchers said that because it is difficult to express directional integration and more random integration in transgenic engineering, the first batch of fluorescent fish can only produce fluorescent effect if they are excited by purple light in dark places. Using Value American researchers are using fluorescent fish as molecular "beacons" to study the early development stages of animals. The Sp2 gene concerned by researchers can regulate the expression of other genes, and the fluorescent fish they created can also provide clues for exploring the causes of tumor development. The research results were published in the recently published journal Biochemistry. Fluorescent fish Sp2 is a member of Sp transcription factor family, and Sp protein plays the role of cell "operon", which can be turned on or off when necessary. Researchers at the University of North Carolina found that the development of skin tumors is positively correlated with the excessive production of Sp2, and other studies have also pointed out similar findings in prostate cancer. But beyond that, people know little about protein. Researchers suspect that the overproduction of Sp2 may be an early indicator of tumor formation. So they implanted fluorescent markers into zebrafish; This marker is linked to Sp2 gene, so they can track the synthesis of Sp2 in the whole organism. Observing zebrafish under ultraviolet light, Sp2 marker will emit red light where the gene is expressed. The researchers said that zebrafish is a good animal model for this study. Because its embryo can develop outward within 24 hours, what happens can be observed under a microscope. In addition, its Sp2 protein is exactly the same as that found in mammals, so it has the same function in humans and zebrafish. Previous studies have shown that Sp2 can regulate development, not only the development of tumors, but also the development of the whole organism. When the research team observed zebrafish, they also quickly discovered the importance of Sp2 for embryonic development. The researchers noticed that in adult zebrafish with fluorescent markers, except for the red fluorescence in female ovaries, there was no light elsewhere, and females could also emit red light when laying eggs, which indicated that Sp2 was very important for the early development stage. Unsurprisingly, after the researchers removed Sp2 from the embryo, the embryo stopped developing. The researchers therefore concluded that they had discovered the basic mechanism of embryonic development. The researchers also believe that fluorescent fish will help them answer another question about Sp2, that is, whether protein also plays an important role in the development of some cancers. For example, if Sp2 plays an important role in the development of brain cancer, researchers can use fluorescent fish to study brain tumors. Theoretically, when the tumor develops, fluorescent fish can also be observed to emit red under ultraviolet radiation. Therefore, these fluorescent fish can also be used as useful tools to detect early tumor development. What should I do if I only buy fish and don't feed fluorescent fish? Basically, no matter what feed is given to the fluorescent fish, the body will still glow and will not fade. And our company's special feed for fluorescent fish has a patented formula, which can enhance the fluorescence brightness and make the color of fluorescent fish more attractive. Life span of fish? Can the life of fish be guaranteed? The habit of the night pearl is exactly the same as that of the green snapper. Even in the wild, it can survive for two to three years, and fluorescent fish are very easy to raise. As long as it is properly raised, the survival rate is extremely high and it will never fade. What are the kinds of miscellaneous fish? Can live in peace with those fish? In principle, don't mix with large carnivorous and predatory fish. Take the fresh water tank as an example. Our common lamp fish, whole fish, zebra, goldfish, peacock fish, octopus and so on. Are appropriate and can be peaceful. Under the long-term irradiation of black light, will it affect the human body? Generally, the visible spectrum is in the range of 400 ~ 700 nm, the black light tube spectrum is in the range of 350 ~ 400 nm, and the ultraviolet light spectrum is in the range of 257nm, so the black light tube spectrum will not cause harm to organisms. We once used a black lamp to irradiate fish for 24 hours for 1 month. As a result, the fish did not appear pathological changes and abnormalities, so it should not cause harm to the organism. Black tubes are often used in stage, mosquito catching lamp, banknote inspection lamp and decoration. There have been no reports of injuries to people, but it is still suggested that it is not advisable to look straight at the black light tube for too long. How to tell the sex of fluorescent fish? The dorsal fin of the male fish is bifurcated, but the female fish is not. The male fish has a long anal fin, while the female fish has a short and round anal fin. Are eggs naturally fluorescent? Because we transfer the fluorescent gene into the germ cells of fish, it can be passed on to future generations through reproduction.