Why should a strain bank be established and how to ensure the quality control of strains?

The strain bank is of great significance, especially the large-scale strain bank recognized internationally and domestically, such as American ATCC. Everyone wants to buy standard strains, and at the same time, if they apply for patents on microbiology, they are also required to keep the strains in the standard strain bank. The publication of new microbial species generally requires that the strains be kept in a large international strain bank, and more and more SCI magazines also require that the strains be kept in an open strain bank before the papers are submitted.

After long-term use and preservation, abnormal phenomena such as low fermentation unit, excessive foam and mycelium deformation will be found, which indicates that the strain is degraded and leads to poor quality. The application of this strain will directly affect the fermentation production, and the quality of the strain is one of the key factors affecting the success or failure of fermentation. In view of the quality problems of strains, combined with the characteristics of strains themselves and the methods and conditions of strain preparation, it is the basis of ensuring stable production to treat degraded strains and restore their original excellent characteristics. This report takes bacteria as an example to discuss.

Under suitable environmental conditions, hyphae can diffuse into the whole culture material after 20-40 days of culture, and can be used for transplanting or producing cultivated seeds after 7- 10 days of culture. If the prepared strain is not used temporarily, it can be stored in a cool, dry, ventilated, dark and clean room for a short time to ensure the quality of the strain.