1, cdna sequence encoding sweet potato phytoene dehydrogenase The present invention provides a cdna sequence encoding sweet potato phytoene dehydrogenase, which is isolated and cloned from the root tuber of red-heart sweet potato. The full-length cdna is 2 18 1bp, and the open reading frame is 1685bp. See table 1 for the detailed sequence. Phytoene dehydrogenase catalyzes the dehydrogenation of phytoene to form phytoene and ζ-carotene in turn, which is the key enzyme in the synthesis of sweet potato β-carotene, so the cdna sequence has important application value. 2. Method for extracting lycopene by microwave radiation A method for extracting lycopene by microwave radiation takes tomatoes or photosynthetic bacteria as raw materials and various organic solvents as extractant. Characterized in that the extraction is carried out by heating and refluxing under the condition of microwave radiation, wherein the microwave radiation time is 5 seconds to 65438 0 minutes, and the power is 65438 000 watts to 800 watts. Lycopene was obtained by saponification, column chromatography and crystallization. 3. The lycopene mixture contains 250.00 mg of natural tomato extract and 65,438 000.00 mg of soybean oil, in which the natural tomato extract also contains 65,438 05.00 mg of lycopene; Phytoene1.50mg; 1.25mg of phytofluene; β -carotene 0.50 mg; Natural vitamin e5.00 mg; Phytosterol 1.50 mg; Phospholipid 25.00 mg; And tomato oil 200.00 mg; Soybean oil can be replaced by pumpkin seed oil. Lycopene mixture is organically cultivated, naturally extracted and pollution-free. 4. A method for preparing lycopene The present invention relates to a method for preparing lycopene. In this method, lycopene is prepared by extractive crystallization. Adding low molecular weight alcohol solvent into lycopene oleoresin under stirring, uniformly stirring, filtering and drying to obtain lycopene, and recovering the solvent from the obtained filtrate by a conventional method. Dissolve fatty acids and their esters in oleoresin with low molecular weight alcohol to promote the crystallization and aggregation of lycopene, and then filter and dry to obtain lycopene fine products. The purity of the refined lycopene product obtained by this method can be controlled between 19-65%, and it can be conveniently used in medicine, health care products and cosmetics. In the whole process, the yield of lycopene is high, the technological process is simple and easy, and the energy consumption is low, which is suitable for industrial production. 5. A method for producing natural lycopene oleoresin and natural lycopene crystals, which comprises extracting tomato sauce with organic solvent, separating and recovering the organic solvent, roughly removing the residual organic solvent, and removing the residual organic solvent by supercritical carbon dioxide to obtain primary or fine lycopene oleoresin. The invention has the advantages of simple production process, less investment, high extraction rate and less organic solvent residue, and is beneficial to large-scale industrial production. 6. A method for rapidly extracting lycopene, which belongs to the technical field of food ingredients. Lycopene is extracted from raw materials rich in lycopene, and after the raw materials rich in lycopene are pretreated, the lycopene is extracted by using ultrasonic/microwave synergistic technology to assist solvent reflux, and the lycopene oleoresin product is obtained after concentration; And the solvent is recovered. The method has simple steps, greatly shortens the extraction time and avoids the loss of lycopene in the extraction process; High extraction rate, up to 97%; A closed system is adopted in the extraction process, so that the solvent consumption is low, the cost is reduced, and the production safety is improved; Using microwave as heating source, energy directly acts on the extraction system, which reduces the waste of energy and has the characteristics of energy saving. 7. The present invention relates to a multi-step process for producing 2,6-cyclolycopene-1, 5- diol, which is an oxidative metabolite of carotenoid lycopene of the following formula. α-terpineol acetate is oxidatively dihydroxylated into cyclohexanediol (Ⅳ), which is oxidatively cracked into ketoaldehyde (Ⅴ), and the aldol in ketoaldehyde (Ⅴ) is condensed into cyclopentanol (Ⅴ), and cyclopentanol (Ⅴ) is silylated into its silylated derivative [formylcyclopentane (Ⅴ)], which makes formylcyclopentane (Ⅴ). A method for producing health product with lycopene as main component. A production method of a health care product with lycopene as the main component is characterized by comprising the following steps: cleaning tomatoes, and mixing the ingredients according to the following weight ratios: ① tomato 90-97kg, ② kudzu root 0.5-1.5kg, and ③ Polygonatum sibiricum 0.65438. 2, respectively pulverizing Radix Puerariae and Radix Astragali, decocting, and filtering; 3. Pulping tomatoes, adding Radix Puerariae solution and Radix Astragali in turn, boiling at high temperature for 1 ~ 1.5 hours, cooling and filtering; Fourthly, concentrating under reduced pressure into 3-5 kilograms of red concentrated juice; 5. Add vitamin C and stir evenly to get the red liquid finished product. The invention retains the biological activity of lycopene, improves the content of lycopene in the product, which can reach more than 85%, has no side effects, and enhances the health care function of the product. 9. A method for extracting and preparing lycopene The invention discloses a method for extracting and preparing lycopene, and Hongyi Li Sina blog belongs to the technical field of food engineering. The method takes tomato or watermelon pulp as raw material and adopts ultrasonic wave and extraction bag technology, including the following steps: raw material treatment and pulping; Solid-liquid separation of fruit pulp; Treating jam with ethanol aqueous solution; Study on extraction of jam with lycopene extractant: and preparation of lycopene crystal. The extraction time of the invention is shortened from the conventional10-20h to10-60min, and the product purity is obviously improved from the conventional10-20% to 70-80%, which has the characteristics of high efficiency, rapidness, easy operation, low cost, high lycopene content and the like, and is suitable for industrial large-scale production of high-purity natural products. /lihongyi 168
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