Because natural hirudin exists in the saliva of bloodsucking leeches? The traditional method of extracting natural hirudin from glands and saliva secreted by them is usually to extract natural hirudin from the head or whole of Japanese medical hirudin.
The conventional extraction method is to take the head of a bloodsucking leech as the raw material, because there are a large number of "pseudo-hirudin" components in leeches with similar structures to hirudin, but they have no biological activity, and the storage and melting of food increase the difficulty of hirudin separation.
Crude hirudin solution was obtained by water-acetone mixture extraction or ethanol fractional precipitation, and then natural hirudin was extracted by chromatography and isoelectric focusing.
2. bionic induction method?
Bionic induction is a method to induce living leeches by bionic induction, so as to obtain leech culture medium. It avoids the one-time killing of leeches by traditional methods, not only protects the wild resources of leeches, but also promotes the development of leech breeding industry, which provides a theoretical basis for the industrialization of hirudin.
The specific method is as follows: Japanese medical leech starved for a week under the condition of oxygenation, and then induced at 25℃ and pH7 for 30 minutes. Make them secrete saliva through an induction system made of pig blood and snails.
Then the saliva is further separated and purified to obtain natural hirudin. This is a revolution in the traditional extraction field of natural hirudin. It won't kill hirudin and can be reused. In the process of separation and purification, the processes of water-acetone extraction or ethanol fractional precipitation are omitted. The operation is simple, the method is unique, the production cost is obviously reduced, and the large-scale production of natural hirudin becomes a reality.
Extended data:
Hirudin can be used to treat various thrombotic diseases, especially venous thrombosis and disseminated coagulation. It can also be used to prevent arterial thrombosis after surgical operation and thrombosis after thrombolysis or vascular reconstruction. Improve extracorporeal blood circulation and hemodialysis process.
In microsurgery, vascular embolization at anastomosis often leads to failure. Hirudin can promote wound healing. Studies have also shown that hirudin can also play a role in tumor treatment.
It can prevent the metastasis of tumor cells and has been proved to be effective for fibrosarcoma, osteosarcoma, angiosarcoma, melanoma, leukemia and other tumors. Hirudin can also be used in combination with chemotherapy and radiotherapy to enhance the curative effect by promoting blood flow in tumors.
References:
People's Network-Nanning High-tech Zone Helps Guangxi Hirudin Expand International Market
? Baidu encyclopedia-hirudin