Patent of foot-and-mouth disease purification

Reply 63263522 Commonly used foot-and-mouth disease vaccine ① Inactivated vaccine Inactivated vaccine is the most widely used vaccine at present, which is safe, reliable and has good immune effect. At present, inactivated vaccine of cell culture virus BEI, inactivated vaccine of O-type foot-and-mouth disease for pigs and inactivated vaccine of O-Asia I for cattle, sheep, camels and deer are mainly used in compulsory immunization of foot-and-mouth disease in China. Among them, the inactivated swine O-foot-and-mouth disease vaccine (II) is a highly effective vaccine made from the virulent swine foot-and-mouth disease strain by BHK2 1 cell proliferation, and the effective antigen content in the vaccine is improved by biological concentration technology. Inactivated virus BEI was emulsified with mineral oil adjuvant, and the injection dose was 2ml/ head, which could resist the attack of 200 minimum disease intensity and the cohabitation infection of pigs. However, inactivated vaccine also has its disadvantages, such as poor immunity; The seed poisons used are highly toxic, and there must be strict prevention facilities and strict operating procedures, which have potential biological safety hazards; The production is difficult and the cost is high; Large dosage and short immune period. ② The research of genetic engineering vaccine has made progress in different degrees all over the world. 1975 Bachrach et al. isolated capsid protein VP 1 from foot-and-mouth disease virus, and made subunit vaccine with incomplete Freund's adjuvant, with good immune effect. Kield et al. (198 1) expressed foot-and-mouth disease virus VP 1 in E.coli for the first time. The experimental vaccine made of the fusion protein can induce cows and pigs to produce neutralizing antibodies. Morgan (1990) and others repeatedly inoculated 58μg of A 12-32 dimer, so that pigs can also resist as a-type attack infection. In recent years, we studied the expression of VPI coding protein of foot-and-mouth disease with T4 bacteriophage as the vector, and detected neutralizing antibodies in guinea pigs' immune tests. On 1994, Fudan University in China successfully developed a genetic engineering vaccine for livestock foot-and-mouth disease and applied for a patent, which has been gradually popularized and applied in China. ③ Synthetic peptide vaccine Synthetic peptide vaccine is a vaccine made by artificially synthesizing protective peptides of pathogenic microorganisms by chemical synthesis, connecting them to macromolecular carriers and adding adjuvants. It is a small peptide containing only immune determinants. Biflle et al. (1982) chemically synthesized 140- 160 amino acid peptide according to the amino acid sequence of FMD virus type O VP 1, which can be coupled with carrier protein to induce neutralizing antibodies to protect guinea pigs. Doel et al. (1990) used 14 1- 158 and 200-2 13 peptide fragments of serotypes A, O and C (FMDV) to form a synthetic peptide with 40 amino acids, which produced specificity for both cattle and guinea pigs. ④ Live vector vaccine is one of the research hotspots in recent years. Usually, non-pathogenic microorganisms are used as vectors to construct recombinant to prepare multivalent vaccines, such as herpes virus, adenovirus, pox virus and non-replicating vectors derived from pox virus. Because foot-and-mouth disease virus is easy to form persistent infection through respiratory tract infection, the development of mucosal immune vaccine is also the focus at present, such as live carrier vaccine with bovine non-pathogenic gonadal virus, salmonella vaccine and mycobacterium tuberculosis vaccine as carriers. ⑤ The research on ⑤⑤DNA vaccine selection began in 1990s, and it is known as the third revolution of vaccine biotechnology. The Mei Island Animal Virus Research Institute of the United States has done a lot of work in the research of DNA vaccines for foot-and-mouth disease, and constructed DNA vaccines with different gene combinations. Experiments on mice and pigs have shown good results, but most laboratories are still exploring. ⑥ Green vaccine Green vaccine refers to genetically modified crops that express antigens and can be eaten raw. Its production engineering construction approach is roughly as follows: firstly, construct plant expression vector containing antigen structural gene, then screen transgenic crops expressing antigen through Agrobacterium-mediated transformation, and then test its immunogenicity through mouse immune response, and the effective one is green vaccine. This study shows that the plant-derived green vaccine can indeed induce mucosal and safe immune responses in animals and humans. Compared with traditional vaccines, green vaccines have the advantages of simplicity, safety, stability, economy and hygiene. View original post >>