(2) The main difference between ④ and ⑤ culture media is that ④ culture media does not add phenol as carbon source, and ⑤ culture media adds phenol as carbon source; ⑥ Single colony can be obtained by stripping coating plate method or plate scribing method. When bacteria are cultured on a solid plate, they should be inverted to prevent water droplets formed after condensation from dripping on the culture medium and polluting the culture medium.
(3) Take five clean culture bottles → add the same culture medium and the same amount of phenol respectively → inoculate five strains with the same amount from different strains respectively → cultivate for the same time under the same suitable conditions → detect the phenol content in the culture medium in these five bottles, so as to compare the phenol degradation ability of different strains.
(4) Sterile operation should be carried out in the whole experimental process from the preparation of culture medium to inoculation culture: aseptic operation generally refers to all methods to prevent contamination by miscellaneous bacteria in the operation of cultivating microorganisms. The key to obtain pure culture is to prevent the invasion of foreign bacteria: a. The experimental operation space, clothes and hands of operators should be cleaned and disinfected;
B. Sterilization utensils, inoculation tools, culture media, etc. used for microbial culture;
C. In order to avoid microbial pollution in the surrounding environment, the experimental operation should be carried out near the flame of alcohol lamp;
D. During the experimental operation, contact between sterile materials and instruments and surrounding objects should be avoided.
So the answer is:
(1) Phenol A plate colony count (live bacteria count or colony count)
(2) (4) Containing other carbon sources except phenol; (5) Use phenol as the sole carbon source for plate separation (dilution coating plate or plate scribing separation) to avoid the moisture generated in the culture process from affecting the growth of microorganisms.
(3) Different strains were cultured in the same phenol concentration culture solution, and the phenol content in the culture solution was determined after a certain period of time.
(4) Sterilization of culture medium, sterilization of inoculation environment and aseptic operation during inoculation.