Florfenicol mainly inhibits peptide acyltransferase by competitively binding to the 50S subunit of bacterial 70S ribosome, thus inhibiting the extension of peptide chain and interfering with the synthesis of bacterial protein.
At present, the detection methods of florfenicol residues include gas chromatography, gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry.
Amphotericin B is almost insoluble in water, and the injection contains cosolvent, usually cholate. The prepared solution is a colloidal solution, which can lead to the precipitation of amphotericin B at low temperature or in electrolyte. You can try to heat the medicine with precipitate with warm water to see if it can dissolve the precipitate again. If not, that's another problem.
Another possibility is that nurses use the same syringe to prepare many drugs. Before amphotericin B was prepared, the drug dissolved in NS was prepared, resulting in a small amount of sodium chloride mixed in the liquid. Because of the small mixing amount, precipitation does not occur immediately, but after being left or used for a period of time.