1 The source of the species must be matched: Select the secondary antibody according to the primary antibody used. Generally speaking, if the primary antibody is of mouse origin, the secondary antibody should be a rabbit/goat or other species anti-mouse antibody. Currently, the American company Vector and Zymed have developed kits that use mouse antibodies to detect mouse tissues. That is, the primary antibodies are derived from mice. The kit uses a universal non-specific blocking solution and a patented blocking endogenous mouse Ig blocking solution can obtain clear staining background.
2. Pay attention to antibody isotypes and subtypes: After determining the primary antibody isotype and subtype, you can choose a broad-spectrum Ig from the source species of the primary antibody or a secondary antibody that targets the primary antibody subtype. If the primary antibody is mouse IgG1, you can use goat anti-mouse Ig or IgG1 secondary antibody.
3. Correct use: You also need to reselect the dilution ratio. Generally, the dilution ratio provided by suppliers starts from 1:10 dilution and is diluted to 5 times.
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