How to identify and screen plant somatic disease resistance by using metabolites of pathogenic bacteria?

The most widely used metabolites of pathogenic bacteria are phytotoxins produced by them, including pure toxins, crude toxins or culture filtrate of pathogenic bacteria. In the early 1970s, Carison first confirmed that plant cell protoplasts can be screened out or disease-resistant plants can be regenerated by using the toxin of pathogenic bacteria.

The toxin screening method is simple and rapid. Wild-type cells are usually sensitive to toxins, and disease-resistant mutant cells can be easily identified and detected. There is usually a good correlation between the resistance of cells to toxins, the resistance of regenerated plants to toxins and the resistance of plants to diseases.

The toxin used for cell identification is preferably a pure toxin with a known chemical structure. Crude toxin and pathogen culture filtrate are not easy to standardize, and may contain many toxin components or other physiologically active substances. If you use crude toxin or culture filtrate as a last resort, you should try to avoid interference from other substances. It is best to treat the cultured cells of known disease-resistant varieties and susceptible varieties in advance in order to know whether there is any identification effect or to find out the available identification characteristics. It is necessary to determine the appropriate treatment concentration, treatment time and screening times through experiments.