Mathematically, a sequence is an object (or event) arranged in a column; In this way, each element is either before or after other elements. Here, the order between elements is very important.
The method of constructing eukaryotic gene knockout library by using CRISPR/Cas9 system is an invention patent filed by Peking University on February 365438+20 13, 2003. The patent application number is 2013107517550, and the publication number is CN65438+. The method of constructing eukaryotic gene knockout library by using CRISPR/Cas9 system is to express the genes encoding Cas9 and OCT 1 protein in eukaryotic cell lines, screen cell lines stably expressing Cas9, and then construct the library and screen the function. Its greatest advantage is that it can be applied to most eukaryotic cell lines and is not limited by specific cell lines. In addition, the positive rate of functional screening is high and the background value is low. Large-scale screening method greatly reduces the cost and overcomes the problem of high time and labor cost caused by single preparation of gene knockout cells. '