1, RT-PCR is real-time? In the case of PCR, just like QCPR, it is a fluorescence quantitative PCR.
2. Compared with ordinary PCR, dyes will be added to facilitate the collection of fluorescence signals.
3. Can the result be relative/absolute? Concentration and other groups of data, and then analyze the actual data according to your purpose.
4. We can also judge whether the experiment is qualified by the ct value of ntc.