1. Moderator: The project "Reverse Genetic System of Porcine Reproductive and Respiratory Syndrome Virus and Development of Genetic Engineering Vaccine" initiated by China Academy of Agricultural Sciences (2005-2008). 4.5 million yuan, under implementation.
2. Analysis of cis-trans regulatory factors of porcine reproductive and respiratory syndrome virus replication.
3. Porcine Reproductive and Respiratory Syndrome Virus IRES
4. Development of replicon expression vector of porcine reproductive and respiratory syndrome virus.
5. Establishment and application of mosaic arteritis virus
6. Nested virus reverse genetic system and genetic engineering vaccine
research results
1. Based on the reverse genetic system of porcine reproductive and respiratory syndrome virus (PRRSV) in North America for which I have intellectual property rights, an infectious clone with the genetic and antigenic characteristics of PRRSV in China was established, and the article is about to be submitted to foreign journals.
2. A series of genetic operating systems have been established, including non-coding region mutation and structural protein overlapping region separation.
August 2000-June 5, 2004 to June 38, 2004+February, 2004, senior research scientist, deputy director (co-director) and senior manager of virus biological products department of ProtaTEK International Company in St. Paul, Minnesota, USA.
Protec International R&D Fund,1000000 USD/year.
1. Moderator: Development of genetic engineering vaccine for porcine reproductive and respiratory syndrome virus.
2. Moderator: Development of inactivated equine influenza virus vaccine
3. Moderator: Develop bivalent inactivated swine influenza virus H3N2/H 1N 1.
4. Main participants: the development of bacteriocin vaccine of swine erysipelas.
5. Object: Establishment of reverse genetic system of bovine parainfluenza virus type 3.
research results
1. Obtained the item of US patent 1:No. US 6,841,342b2 "Porcine Reproductive and Respiratory Syndrome Virus Infectious cDNA Cloning and Expression Vector Treatment" as the first completion person.
2. The first person to complete the world patent (PCT) application of the same name (submitted in March 2002). Under review.
3. American patent application 1 "Recombinant vacuum against porcine reproductive and respiratory syndrome virus" (filed in February 2004, 65438) was the first person to complete it. Under review.
4. The first person to complete the veterinary vaccine production license issued by the US Department of Agriculture "Inactivated vaccine of equine infection virus strain Kentucky 98". Univalent vaccine and EIV/EHV- 1 combined vaccine cooperated with Pfizer have been put on the market.
5. The second finisher of USDA's application for veterinary vaccine production license 1 "Vaccine for preventing swine erysipelas infection" (submitted in June 5438+February 2004). Under review.
August1996-April 2006 5438+0, Ph.D. Assistant Research Fellow, College of Veterinary Medicine, University of Minnesota, USA.
1. Joint Principal Investigator: RNA recombination and genetic variation mechanism of porcine reproductive and respiratory syndrome virus.
2. Co-principal investigator: Identify novel subgenomic RNA molecules related to porcine reproductive and respiratory syndrome virus.
research results
1. during this period, * * * published 1 1 articles in SCI international journals, among which 6 articles were signed by the first author.
2.4 Patent and Intellectual Property Application
Won the Young Paper Award of the Annual Meeting of the American Society of Virology for 3.3 times.
4. 1 Young Scientists Award of International Conference on Honeycomb Virus
5. Dean Award of College of Veterinary Medicine, University of Minnesota 1 time.
6. The Center for Edible Animal Biotechnology of the University of Minnesota awarded three doctoral students the annual Excellence Award.
65438+August 0990–65438+July 0996, and was promoted to associate researcher on 1995. Research Project of State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
1. Moderator: Key Biotechnology Project of the Ministry of Agriculture during the Eighth Five-Year Plan. Animal gene engineering expression vector and bioactive preparation >:>( 1992-1995), funded by 320,000 yuan, completed and passed the appraisal of the Ministry of Agriculture.
2. Moderator: Research and application of avian pox virus vector, a sub-project of the Eighth Five-Year Plan of the Ministry of Agriculture (1992-1995), supported by150,000 yuan. It was completed and appraised by the Ministry of Agriculture.
3. Acting Moderator: The general project of National Natural Science Foundation of China "Screening and Identification of Non-essential Gene Fragments of Fowlpox Virus Replication" (1992-1995, moderator: Xu Zhendong), with a fund of 70,000 yuan, passed the appraisal of the Ministry of Agriculture.
4. Main participants: the National Eighth Five-Year Plan key project "Cloning and Expression of Bovine Interleukin -2" (199 1- 1995, hosted by Yu), supported by180,000 yuan.
5. Main participants: National key project "Epidemiological investigation of highly pathogenic avian influenza virus" during the Eighth Five-Year Plan period (1991-kloc-0/995, moderator: Yu),120,000 yuan.
research results
1. During this period, * * * published 10 articles in national core journals, including 4 articles signed by the first author and 5 articles written by the correspondent.
2. Won 6 ministerial-level achievements appraisal by the Ministry of Agriculture, including 2 first finishers.
3. Won excellent papers at provincial and ministerial level 1 article, signed by the first author.
4. Received 5 excellent papers from China Animal Husbandry and Veterinary Society and chinese society for microbiology Annual Meeting, including 2 first authors and 2 corresponding authors.
5. 3 international conference exchange papers, 2 signed by the first author;
6. Won the title of advanced scientific and technological worker of China Academy of Agricultural Sciences 1994.
7. Instruct 3 graduate students; Assist in guiding two doctoral students; Instruct five postdoctoral and visiting scholars.
65438+August 0987–65438+July 0990, Master. Research Project of Veterinary College of Nanjing Agricultural University/State Key Laboratory of protein and Plant Genetic Engineering in Peking University
1. Isolation and Identification of Rabbit Hemorrhagic Disease Virus
2. Serological investigation of hepatitis B virus infection in dairy cows.
research results
1. 2 articles published in domestic core journals, signed by the first author.
2. Second Prize of National Science and Technology Progress (1 Du Nianxing 2 Xu 3 Gong Xiaoming 4 Ji Chuanyi 5 6 Li Rujian 7 Wu Yaomei Zhou Yang Deng Jinquan Shun Zheng Feng)
3. The first prize of the Ministry of Agriculture (1 Du Nianxing 2 Xu 3 Gong Xiaoming 4 Ji Chuanyi 5 Li Rujian 6 Wu Yaomei 7 Zhou Yang Deng Jinquan Shun Zheng Feng)
4. 1 articles exchanged at international conferences
5. 1 Excellent Paper Award of chinese society for microbiology Annual Meeting.