The rapid detection technology system of immune test paper was established.
It provides technical support for rapid detection of major animal diseases and animal-derived food safety in China.
1 The infectious bursal disease in chickens was systematically carried out.
Study on rapid detection technology of major animal diseases such as Newcastle disease, avian influenza and swine fever. A new preparation method of paired monoclonal antibodies with high specificity and affinity was established, which solved the key technical problems such as large variation of animal virus antigen, low affinity of antibody and narrow recognition spectrum in the development of test paper. The rapid detection technology system of antigen immune test paper was established, and a series of products of rapid detection test paper for animal disease antigen were developed, which opened up a new field of rapid diagnosis of animal diseases and provided new technical support for rapid detection of major animal diseases. In 2003, the "Test Strip for Rapid Diagnosis of Livestock and Poultry Diseases" won the national invention patent (ZL991KLOC-0/537.1), among which the "Test Strip for Rapid Detection of Infectious Bursal Disease Virus" was the first test strip product for rapid detection of animal diseases at home and abroad, and in 2003, it won the second class of new veterinary drug certificate [(2003
2. The rapid detection technology of small molecule drugs was systematically studied.
The preparation and identification methods of small molecular drug artificial antigen and high specificity and high affinity monoclonal antibody were established, which solved the key technical problems of low immunogenicity, strong cross-reaction and low affinity of small molecular drug. The rapid detection technology system of hapten immunoassay test paper was established, and a series of rapid detection test paper products of five categories 15 kinds of illegal drugs were developed, which realized the simple and low-cost rapid detection of drug residues and provided technical support for the safety monitoring of animal-derived foods. This research field has applied for patents 16, and 9 patents have been authorized. In 2008, the "Rapid Detection Technology of Main Prohibited Drug Residues in Pigs by Immunoassay Paper" won the second prize of National Science and Technology Progress Award.
3. The rapid detection technology of antibody was studied systematically.
The preparation methods of pathogen detection antigens such as foot-and-mouth disease virus, classical swine fever virus, Newcastle disease virus and Trichinella spiralis were established, which solved the outstanding problems of low activity, high non-specific reaction and difficult extraction and preparation of immunodetection antigens. The rapid detection technology system of antibody immune test paper was established, and a series of products for rapid detection of antibodies against animal diseases and zoonosis were developed, which realized real-time monitoring of antibody levels against animal diseases and provided advanced technical means for vaccine immune effect evaluation and animal disease monitoring. Among them, the "Test Strip for Rapid Detection of Trichinella Antibody in Swine" won the second prize of National New Veterinary Medicine Certificate [(2007) No.36] in 2007, and won the first prize of Henan Science and Technology Progress Award in 2008.
The above research has established a rapid detection technology system for antigens, haptens and antibodies, including six technical modes. Most of the rapid detection test paper products for animal diseases and zoonosis and the rapid detection test paper products for animal-derived food safety are the first in the world, and the detection time only takes a few minutes, without any additional reagents and equipment, and everyone can operate them. It has achieved the long-sought goal of "rapidity, simplicity, specificity and sensitivity" in the field of detection technology, solved the problems of time-consuming (hours to days), high cost (expensive instruments or reagents) and complicated operation of traditional detection methods, improved the research and development level in this field in China, and promoted the progress of immunoassay technology.
Secondly, the Fc receptor of animal immunoglobulin was studied systematically.
1 animal immunoglobulin was studied systematically.
Molecular characteristics and immune function of (IgG) Fc receptor, and established affinity molecular cloning method based on rose ring indicator system. There are 1 1 IgG Fc receptor molecules in pigs, cattle and sheep in the world, of which 10 was discovered by the research team in Zhang Gaiping. It was found that there were IgG Fc receptors in bovine dendritic cells, which were characterized as FcγRII, thus IgG Fc receptors in human and mouse dendritic cells were found. A new Fc receptor (boFcγ2R) was found in ruminants, which explained the hyperimmunity of ruminant IgG2 discovered by Waltson (1976) and denied the inference of Symons et al. (1989) that bovine IgG2 could not have Fc receptor. The molecular mechanism of antibody-dependent enhancement (ADE) of porcine reproductive and respiratory syndrome (PRRSV) virus infection was revealed, and a new method for detecting ADE infection of PRRSV was established, which is of great significance for preventing and controlling this major epidemic disease.
2 The animal IgG Fc receptor was systematically studied.
The molecular mechanism of interaction with ligands shows that there are linear epitopes binding to ligands on bovine IgG2 Fc receptor, and epitope peptides can effectively regulate the interaction between IgG and Fc receptor. Because Fc receptor can mediate autoimmune diseases, Zhang Gaiping's research team found the linear ligand binding epitope of human Fc receptor on the basis of the above research. Experiments on systemic lupus erythematosus (SLE) model mice prove that human Fc receptor epitope polypeptide can slow down the pathological process of SLE mice and effectively reduce their death, which provides a new way for developing new drugs to treat difficult diseases such as human autoimmune diseases. Apply for two invention patents in this research field.
Thirdly, the immune recognition of animal viruses is studied.
The research platform of animal virus immunohistochemistry was established, and the B cell recognition map of main animal virus proteins was drawn.
A series of B cell recognition sites of classical swine fever virus, infectious bursal disease virus, Newcastle disease virus, avian influenza virus and other major animal virus proteins were found, and 12 B cell epitope sequences were registered in GenBank, and the B cell recognition maps of major animal virus proteins were drawn, revealing the humoral immune mechanism of major animal virus diseases and enriching the theory of animal virus immunology. Five invention patents have been applied for and three have been authorized in this research field.
Over the years, Zhang Gaiping and his team have carried out innovative work in the fields of rapid detection and research of animal diseases and animal-derived food safety, and presided over 22 major national scientific and technological projects. As the first person to complete, he won 2 national science and technology awards and 3 provincial and ministerial science and technology awards; Published research papers 147, including 22 SCI papers, which were cited by famous journals such as Science 12 1 time; 36 patents were applied and 4 patents were granted/kloc-0.
Humana Publishing Group of the United States specially invited Zhang Gaiping to write "lateral flow test strip for immunochromatography" for the book Biosensor and Biological Detection (ISBN: 978- 1-60327-568-2, 2009), which is the first time that this technology has been officially used.