Development and utilization of Pichia pastoris

Koichi Ogata et al. first discovered in 1969 (Ogata, et a 1. 1969) that some yeasts can grow with methanol as the sole carbon source and energy source. Since then, the potential of using methanol yeast to produce single cell protein as animal feed has attracted wide attention. 1987, Clegg and others first reported the expression of hepatitis B surface antigen by methanol trophic yeast (HbsAg), and then Philip Oil Company and Salk Institute Biotechnology/Industry Joint Company (SIBIA) began to cooperate to develop Pichia pastoris expression system. Sibillat. The researchers isolated the promoter and host strain of AOX gene, constructed the vector and developed the corresponding gene manipulation technology of Pichia pastoris. Combined with the fermentation process of producing single cell protein by Philip Oil Company, the high-efficiency expression of foreign protein was realized. 1993, Philip Oil Company sold the patent of Pichia pastoris expression system to Research Corporation Technologies Company and entrusted Invitrogea Company to sell related products.