One is different charges based on GHB and non-GHB, such as ion exchange chromatography, electrophoresis and isoelectric aggregation.
The second is based on the structural characteristics of glycosylation groups on hemoglobin, such as affinity chromatography and immunoassay.
(1) Affinity chromatography: This method has strong specificity and is not interfered by abnormal hemoglobin. Suitable for clinical departments, especially children. The test results also completely meet and exceed the clinical requirements, and the CV value is less than 5%. Take NycoCard READER II, a Norwegian cyclone glycosylated hemoglobin instrument of Zhongxing Yike (Beijing) Trading Company as the representative.
(2) High-pressure liquid chromatography can automatically separate and determine the variants and subtypes of glycosylated hemoglobin and hemoglobin, but the operation and maintenance requirements of the instrument are high, and the CV value is less than 65438 0%. Represented by HLC-723 G7 of Beijing Zhixin Medical Company.
(3) Immunoagglutination method: The principle is that glycosylated hemoglobin will agglutinate after binding with the corresponding monoclonal antibody. The agglutination amount is expressed by measuring absorbance, which can be measured by automatic biochemical analyzer. Use a new kit for each test, and pay attention to mixing reagents before operation. It is pointed out that the precision of measuring glycosylated hemoglobin by immunoagglutination method is poor, and the CV value is generally not more than 5%. Acting by DCA Vantage of Zhong Yi Kang Hui (Beijing) Trading Co., Ltd.
(4) Ion trapping method, which is based on the principle that glycosylated hemoglobin is combined with corresponding antibodies and then connected with fluorescent markers. Polymer tetramine is coated on glass fibers in IMX reaction holes in advance, so that the fiber surface is positively charged, and the above reaction complex is adsorbed on the fiber surface. After a series of cleaning, the fluorescence intensity is measured, so as to obtain the concentration of glycosylated hemoglobin. This method is suitable for the detection of bulk glycosylated hemoglobin samples. Represented by IMX of Jingdongnan Chemical Industry.
(5) Electrophoresis: Capillary electrophoresis can also separate and detect glycosylated hemoglobin and hemoglobin variants, but there is no commercial instrument with batch sampling ability at present, which limits the clinical application of this method to a certain extent.