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Four kinds of precious stones have fewer natural precious stones than Huang Jingui. Gallstones in cattle are natural bezoar, which is more expensive than gold. The annual demand for bezoar in China is about 5 tons, but the self-produced natural bezoar is less than 1 ton.
Calculus bovis cultured in vitro is made from fresh bile of cattle as mother liquor, and deoxycholic acid, cholic acid and compound calcium bilirubin are added. In vitro culture of calculus bovis is is the principle and biochemical process of simulating the formation of gallstones in cattle. Fresh bile of cattle was used as mother liquor, and deoxycholic acid, cholic acid and calcium bilirubin complex were added to make calcium bilirubin stone. Its bilirubin content is limited to not less than 35.0%.
Implant foreign bodies into the gallbladder of cattle, and then suture the wound. Cattle secrete special substances to wrap foreign bodies. After a long time, foreign bodies will be surrounded by calcium salts and become organic substances, forming artificial gallstones. Manual preparation: cytochrome 0.7%, cholesterol 2%, bovine and sheep cholic acid 12.5%, cholic acid 15%, inorganic salt 5%, magnesium sulfate10.5%, ferrous sulfate 0.5%, tricalcium phosphate 3%, and the rest is starch.