NycoCard Reader II multifunctional full-quantitative gold label detector is produced by Nycor company in Norway. This instrument is small in size and easy to carry. The test system consists of two parts: the instrument itself and the reagent board. There is a data display screen on the dashboard for operation tips, and there are three operation keys at the bottom of the screen. With these three keys, the whole process of project selection and testing can be completed. There is an optical probe at the upper right of the instrument, which is used for blank calibration and quantitative detection of samples in the reagent plate.
The test is simple and convenient: generally 3-4 steps can complete the rapid quantitative detection. The sample collection is small and convenient, and complicated pretreatment is not needed. The full quantitative result report is accurate and fast, and the detection is completed in 3 ~ 5 minutes.
Full-featured, one machine can do many things. According to different requirements, different detection procedures and matching reagents can be selected to determine CRP, HbA 1c, U- albumin and D- dimer. The advanced nature of the project and the practicality of diagnosis and treatment show excellent clinical value.
Equipped with quality control to monitor the stability of instrument reagents.
Instrument reagents have passed the certification of FDA, CE, SFDA, NGSP, DCCT and other authoritative organizations.
Excellent cost performance, the best of its kind.
Supplier: Zhongxing Yike (Beijing) Trading Co., Ltd.
2. Introduction of 2.NycoCard Reader II test project.
Characteristics of CRP determination
(1) the principle of solid-phase immune double antibody sandwich method uses specific monoclonal antibodies. When the diluted sample flows through the reaction membrane, CRP is captured by the specific monoclonal antibody fixed on the membrane, and then combined with the added colloidal gold antibody conjugate, and the unbound gold labeled antibody is removed by the washing liquid. The purplish red intensity on the membrane is directly proportional to the CRP concentration, which can be quantitatively detected by the instrument within 5 minutes.
⑵ The fixed-value quality control provided with the kit is a reference corrected by CRM470(IFCC/BCR/CAP).
⑶ Anti-interference: There is no cross reaction with other blood components, especially bilirubin, lipid or rheumatoid factor (RF) due to the application of anti-human CRP specific monoclonal antibody. It can avoid the interference of liquid turbidimetry on the detection results due to lipid turbidity, impurity protein, RF and other factors.
⑷ 5ul micro-specimen, which is universal for whole blood, serum and plasma specimens. Ultra-wide detection range (8-250 mg/L) precision CV
⑸ Cause analysis of CRP "rising" at present.
It has been more than 70 years since American scholars named CRP from 65438 to 0930. In recent years, the experiment has obviously "warmed up" and attracted much clinical attention. This is because:
A) The increase of drug-resistant strains is a very difficult problem at home and abroad. An important cause of drug-resistant bacteria is the abuse of antibiotics, regardless of whether the bacteria are infected by viruses or not. CRP detection can provide powerful help to distinguish bacterial infection from viral infection, guide doctors to use antibiotics in a targeted manner, and play an important role in preventing the emergence of drug-resistant bacteria. In addition, CRP detection has very good clinical value in evaluating the efficacy of antibiotics, prompting disease activity and predicting postoperative complications.
B) With the improvement of detection technology, from qualitative detection in the past to real-time quantitative detection report mode, specific data comparison is more valuable for clinical diagnosis.
C) CRP is more sensitive to progressive diseases than WBC and ESR. The normal range of white blood cells is too large. In some patients with poor reactivity, even with bacterial infection, WBC is normal and CRP can be significantly increased.
D) A new viewpoint that CRP is closely related to cardiovascular diseases has been put forward abroad, and its harm even exceeds the increase of blood lipid. This revolutionary breakthrough to the traditional theory makes people pay more attention to the detection of CRP.
Characteristics of HbA 1c determination
⑴ Using the principle of chromatography affinity chromatography, the different intensities of blue (HbA 1c) and red (total Hb) of reagent plate reaction were measured with Reader II, and the percentage of HbA 1c in the sample was calculated. No matter the concentration of hemoglobin is high or low, the percentage relationship will not change, and the reporting method of glycosylated serum albumin umol/L may be affected by the increase or decrease of serum albumin caused by non-diabetic diseases.
⑵ Specificity: Glucose, bilirubin, lipid, fructosamine and abnormal hemoglobin were added to the sample, and no interference was found.
⑶ Quality control: provided by Axis-shield and POCAS/Lccal (not included in the kit).
⑷ Reference standard: refer to the standards recommended by DCCT in the United States and the reference laboratory of glycosylated hemoglobin in Europe.
Quality control and standardization of saccharification are issues that people are generally concerned about. At present, the most important problem about HbA 1c detection in the world.
The problem is the lack of a standardized scheme that can be widely recognized. Especially because there are many variants of hemoglobin, there is no clear definition of the analysis object at present. In order to end this situation, the HBA1C standardization working group of the International Standardization Committee (IFCC) decided to adopt (? -n-( 1- deoxyfructosyl) hemoglobin) was used as the determination standard.
Before establishing a scientific and reasonable reference system, american association for clinical chemistry (AACC) Glycosylated Hemoglobin Standard Sub-committee and IFCC HbA 1c Standardization Working Group suggested that the method used in the study of American Association for Diabetes Control and Complications Investigation and Detection (DCCT) should be used as the designated comparison method. At present, the experimental methods of HbA 1c tester produced in various countries should refer to this specified comparison method, and the technical parameters of the instrument are marked with "DCCT standardization".
The most authoritative glycosylated hemoglobin testing institution in the world, the National Reference Laboratory of the University of Missouri, USA, accepts the regular delivery of FDA-approved instruments in various countries and publishes the test results. The test results of Reader II and other brands of instruments can be found on its website.
Clinical application of glycosylated hemoglobin (HbA 1c);
The auxiliary diagnostic index of diabetes screening is more sensitive than fasting blood glucose. Not affected by diet and drugs.
It can reflect the overall situation of glucose metabolism in the past two months and understand the disease control situation from a macro perspective.
Predict the risk of complications in diabetic patients;
The increase of HbA 1c is of great significance in predicting diabetic complications, especially early diabetic nephropathy and retinopathy.
Differential diagnosis of non-diabetic hyperglycemia;
All kinds of stress, such as myocardial infarction and coma patients who are receiving glucose, can increase blood sugar, but HbA 1c is normal. If it is diabetes, HbA 1c will increase. Both of them are of great value in differential diagnosis and treatment.
Application of hyperglycemia during pregnancy;
In the first three months of embryonic development, the level of HbA 1c in pregnant women is very important for the healthy development of the fetus.
Determination characteristics of urinary microalbumin
⑴ Detection principle: solid-phase immunoassay, the surface of reaction plate membrane is coated with specific anti-microalbumin monoclonal antibody, and the principle is the same as CRP determination.
⑵ Specificity: NycoCard Reader II kit uses specific monoclonal antibodies against human albumin, and other components in human urine samples, such as glucose (50mmol/L), creatinine (60mmol/L), nitrite (10mmol/L), NaCl(500mmol/L), ketone body (2.4g/L), etc. Microglobulin (250 mg/L), bilirubin (50 mg/L) and urea (20 g/L) do not cross-react. The PH value has no interference to this experiment.
⑶ Standardization: nyco card U- albumin detection system is calibrated according to the international urine standard CRM470(IPCC/BCR/CAP reference).
⑷ measuring range: the measuring range of nyco card U- albumin is 5-200mg/L.
5. Precision: the coefficient of variation (CV) of NycoCard U- albumin kit is 5 ~ 8%.
[6]Reader II instruments HbA 1c and U- Lu Min were designated by the "Comprehensive Prevention and Treatment of Diabetes" project of the Ministry of Health.
Clinical application of urinary microalbumin;
Normal value: urinary microalbumin < 30mg/L (maximum 24 hours)
1) Hypertension with elevated U- albumin can be used as an index to understand the degree of renal injury and predict it. It is suggested that doctors take effective measures to lower blood pressure to slow down and interrupt the damage of tiny blood vessels.
2)U- albumin is a sensitive index of vascular diseases, which is more sensitive than fundus examination.
3) The application value of diabetes:
It can predict the development and early diagnosis of diabetic nephropathy.
The earliest clinical signs of diabetic nephropathy are the continuous increase of urinary albumin excretion rate and microalbuminuria.
U- albumin-LT20 mg/L is normal proteinuria.
The excretion rate of U- albumin is 20-200 mg/L, which is microalbuminuria with negative urine protein. It can be clinically diagnosed as early diabetic nephropathy. At this time, the lesion is in a reversible stage. By controlling blood sugar and using appropriate drugs, the possibility of diabetic nephropathy can be reduced or interrupted, and U- albumin can be restored to normal.
U- albumin & gt200 mg/L, urine protein quantitative routine >: 0.5g /24h, diagnosed as diabetic nephropathy,
Renal function damage has entered an irreversible stage.
U- albumin is a sensitive index of diabetic microvascular complications, and U- albumin is higher than >; 30 mg/L, the excretion rate is related to the severity of the disease.
Characteristics of determination of D- dimer
1) Methodological principle: monoclonal antibody immunofiltration gold standard method, recommended by Professor Cong Yulong in his book "Clinical Laboratory Science Today" (P 155).
2) Specificity: Monoclonal antibody against D- dimer epitope was used. It is only specific for D- dimer, a degradation product of fibrin, and will not cross-react with other components in plasma, such as fibrinogen and fibrin monomer.
3) Each kit provides quality control.
4) Strong anti-interference, and the existence of high-value bilirubin and hemoglobin does not affect the determination; However, blood cells or platelets in plasma samples will interfere with the results; So it needs to be removed by centrifugation.
Clinical application of d- dimer;
Normal d-dimer
This index can be used not only for the diagnosis of thrombotic diseases, but also for monitoring the therapeutic dose of thrombolytic drugs and observing the curative effect.
Nycocard Reader multifunctional full quantitative gold calibrator is being upgraded and expanded. On the basis of the original four items, the new generation of Afinion instrument has added highly sensitive CR P(HsCRP), homocysteine (Hcy), urinary creatinine ratio (ACR), PT and so on. The method is simpler and faster; The results are accurate and stable.
Supplier: Zhongxing Yike (Beijing) Trading Co., Ltd.