Mechanism of bioleaching
Bioleaching technology uses Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans to dissolve insoluble heavy metals in sludge from solid phase to water phase, and then removes them through solid-liquid separation. It is generally believed that Thiobacillus ferrooxidans has two mechanisms to dissolve heavy metals in sludge.
1. Direct mechanism: The bacteria directly adsorb the metal sulfide (MS) on the sludge surface through the extracellular polymer (EPS) secreted by themselves, and directly oxidize the metal sulfide through the unique oxidase system in the cell to generate soluble sulfate.
Second, indirect mechanism: mainly using the metabolite of Thiobacillus ferrooxidans-ferric sulfate and metal sulfide for redox reaction, ferric sulfate is reduced to ferrous sulfate and generates elemental sulfur, and the metal is dissolved in the form of sulfate, while ferrous is oxidized to trivalent iron by bacteria, and elemental sulfur is oxidized to sulfuric acid by bacteria, forming a redox circulation system. Through bioleaching, the pH value of sludge was reduced to about 2.0, which greatly promoted the dissolution of heavy metals in sludge. The reaction equation is as follows:
2fe2++1/2o2+2h +→ 2fe3+H2O (ferrous oxidizing bacteria such as thiobacillus ferrooxidans and thiobacillus ferrooxidans).
MS+2Fe3+→M2++2Fe2++S (chemical oxidation)
2S+3O2+2H2O → 2H2SO4 (sulfur oxidizing bacteria, such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans)
Steps of bioleaching method
In order to obtain higher heavy metal removal effect and shorten residence time, the following five steps are often adopted in practical application or continuous operation process design:
Firstly, the sludge (solid content is 2% ~ 10%) is pre-acidified to pH4.0 (it has been reported recently that it can be successfully achieved without pre-acidification);
Secondly, adding energy substances, such as FeSO 47 H2O (5 g/L ~ 20 g/L sludge) and elemental sulfur (5 g/L ~ 10 g/L sludge);
Thirdly, adding inoculum, stirring and refluxing sludge (reflux rate is 10% ~ 20%), and culturing for several days to weeks under aerobic conditions;
Fourthly, filtering and dehydrating the detoxified sludge; Fifth, the dehydrated sludge is neutralized with lime and then used in agriculture.