Advantages of loop-mediated isothermal amplification method: high sensitivity (2 ~ 5 orders of magnitude higher than traditional PCR method); Short reaction time (30-60 minutes to complete the reaction); Clinical use does not require special instruments (real-time turbidimeter is recommended in the development stage of the kit); The operation is simple (whether it is DNA or RNA, the detection step is to mix the reaction solution, enzyme and template in the reaction tube, put it in a water bath pot or thermostat at about 63℃ for 30 ~ 60 minutes, and observe the results with naked eyes).
Disadvantages of loop-mediated isothermal amplification method: high sensitivity, easy to form aerosol pollution once the cover is opened, and the problem of false positive is serious because most laboratories in China can not strictly partition at present. Therefore, we strongly recommend using real-time turbidimeter in the development of the kit, and don't open the reaction tube after the reaction; Primer design requirements are relatively high, and genes of some diseases may not be suitable for loop-mediated isothermal amplification.