Difference between horizontal in-situ hybridization under light microscope and horizontal in-situ hybridization under electron microscope

The difference between in-situ hybridization at light microscope level and in-situ hybridization at electron microscope level lies in the difference of probe labeling and detection methods. The method of determining the position of specific nucleotide sequence in chromosome or cell by molecular hybridization with labeled nucleic acid probe is called in situ hybridization. The difference is that the probe marks are different. In-situ hybridization under electron microscope uses some small biological molecules, such as biotin, instead of radioisotope or fluorescein labeled probes in light microscope. At the same time, the detection methods are also different. The detection of hybridization reaction under electron microscope is displayed by colloidal gold particles connected with avidin antibody, while the existence of nucleic acid is directly displayed under optical microscope.