Application of yolk antibody IgY in disease diagnosis

IgY is an ideal tool for immunological experimental detection, which can not only prepare specific antibodies against specific pathogenic organisms for pathogen isolation and identification, but also does not bind with rheumatoid factor, protein A, protein G, mammalian FC receptor and complement, and has almost no cross reaction with IgG. Therefore, it is applied to the specific determination of circulating immune complex, rheumatoid factor and complement, which effectively reduces the occurrence of false positives. In preparing specific antibodies for diagnosis, Yuri Motoi and others successfully prepared specific IgY for rabies virus diagnosis. The recombinant rabies virus CVS- 1 1 chain was expressed in Escherichia coli by gene recombination technology, and was used to immunize laying hens. Immunological method showed that IgY was well bound to the virus and had high sensitivity. Dr. Lonardo and others made a comparative experiment with chicken IgY and rabbit serum antibody IgG, and found that IgY could recognize epitope more effectively than IgG in Western blot and immunoprecipitation reaction. In immunocytochemistry, only IgY can specifically locate E7 protein (the main transporter of human papillomavirus) in HPV 16 positive cells. Terzdo and other experiments show that the specificity IgY for Salmonella hemorrhagic enteritis is high and the effect is ideal.