1994-65438+In February, the first national leaf protein seminar was held in Changsha. Under the guidance of the CPC Central Committee and the State Council on strengthening scientific and technological innovation, developing high and new technologies and realizing industrialization, the application value of Pinus sylvestris var. mongolica was recognized and supported by 35 experts and professors at the National protein Conference.
? Studies by experts and professors in the fields of biomedicine and chemical industry show that protein products directly extracted from fresh stems and leaves of Pinus sylvestris var. mongolica are non-toxic and harmless natural high-grade nutritious foods. The high content of superoxide dismutase (SOD) in slash pine has health care function and pharmacological action, and SOD medicine capsule has a very significant clinical effect. Therefore, the research and development of leaf protein (LPC) and superoxide dismutase (SOD) of Pinus sylvestris var. mongolica was listed as the national key project of high-tech industrialization in 200 1 year by the State Planning Commission and the Ministry of Science and Technology.
In April 2004, the National Development and Reform Commission, the Ministry of Science and Technology and the Ministry of Commerce jointly formulated the Guide to Key Areas of High-tech Industrialization with Current Priority. Article 3 1 of the Guide clearly points out that the key technologies and main products of fermentation engineering, including superoxide dismutase, are included in the national 11th Five-Year Plan. It can be seen that the rapid development of SOD series products is an urgent need to implement the "Eleventh Five-Year Plan" and accelerate the development of high-tech industries.
From 65438 to 0995, Qin Heyuan, a popular science enthusiast in Yuanjiang City, Hunan Province, successfully planted Pinus sylvestris var. mongolica, and made an in-depth study on leaf protein and SOD. 1997 On July 2nd, the People's Daily reported Yuan Guobi, the leader of the national collaboration group of Pinus sylvestris var. mongolica. Pinus sylvestris and Institute of Botany, Chinese Academy of Sciences, said in response to a reporter's question that Pinus sylvestris var. mongolica. Polygala tenuifolia has the characteristics of fast growth, strong regeneration and high nutritional value. Due to the influence of mad cow disease, the EU banned the use of animal SOD from 1997 to 1, and experts and scholars at home and abroad turned their attention to the field of plant SOD. The development and research of the company began to show its importance. In recent years, SOD in plants in China? There are many related progress reports in the research field. Among them, Professor Qin Heyuan, the pioneer of national industrialized production technology of plant turf? After 10 years of research, superoxide dismutase (SOD) was successfully extracted from Pinus sylvestris var. mongolica, which fundamentally overcame the cross-infection of animal superoxide dismutase virus. June 4, 2002 165438+ The research achievement has passed the appraisal of Hunan Science and Technology Department, and it is considered that the research project is "the first in China, and the leading level in China." The application and development of plant SOD has become the mainstream in the field of SOD.
Pine grass, also known as China grass and French champagne grass, is a high-quality natural raw material for both medicine and food, and is a traditional herb of North American Indians. M Grieve's Modern Herbology reported: "The root of this herb can be used as medicine, as a tonic or tonic, which has the functions of anti-fatigue, strengthening physique, diuresis, sweating and tonifying deficiency". The content of protein in hay is 17.83 ~ 22. 15, the content of leaf protein is 77.3%, the active unit of S·O·D in each gram of hay is 2.85 U, the content of flavonoids is 2.86m·g, and the amino acid composition is reasonable. Its herbs are effective for intractable cough, asthma, lung, liver, internal injuries and ulcers. And often used as traditional herbs for bathing, fumigation, cold and other health care and disease treatment. In addition, it is also used as raw materials for medicinal liquor, medicinal latex and chewing gum. The biggest advantage of its grass itself is its rich protein and reasonable amino acid composition, second only to soybean. In particular, lysine and superoxide dismutase (SOD), two restrictive amino acids, are extremely high in content, so their biological value is very high.
Headed by Zuo Quan, chief expert of National Laboratory of Immunodiagnostic Reagent Engineering, Hong Kong Molecular Biology Laboratory and Puji Biomedical Group, the core technology research team organized by Hunan Food Source Biotechnology Co., Ltd. has been seriously studying the extraction technology of effective components of various natural products after ten years of hard work, drawing on and absorbing the most advanced international design concepts, and innovating the two-phase enzymatic SOD extraction and enzyme immobilization modification technology with independent intellectual property rights. In terms of process expression, repeated experiments and continuous innovations in the details of each process step and continuous design and optimization of process structure can effectively control and reduce industrial costs. The technology of this project has the characteristics of safety and environmental protection (no pollution), large capacity (industrialization), high efficiency (high extraction rate) and high quality (high purity and high specific activity).
It has an important breakthrough and unparalleled advantages in the extraction technology of the same industry. Two national invention patents have been applied: a method for extracting superoxide dismutase (SOD) from plants (Shenzhoucao); the patent application number is ZL 201310164284.3. At present, super-standard biological products (drugs) with high purity and high specific activity have been prepared by molecular modification and enzyme modification techniques (ultra-ten-million-level Cu/Zn-SOD freeze-dried powder), and the specific activity of Cu/Zn-SOD has reached 29 million (2.9× 10< U/g), which has been tested by Guangzhou Analysis and Testing Center, a national authoritative organization, with the report number of 20/kloc. Patent has been applied: a preparation method of a new microsphere biological material coated with SOD active drug carrier (20 16 10358799.0). It provides scientific research for the development of medicinal enzymes and therapeutic enzyme preparations in China and fills the application gap. SOD has been successfully extracted from a variety of plants (see the figure below and the test report of China Guangzhou Analytical Testing Center, an authoritative national testing institution).
At present, the activity and specific activity of superoxide dismutase (SOD) extracted from plants can hardly exceed 3 million international units. The plant SOD products produced by two-phase enzymatic extraction and enzyme immobilization modification technology were identified by China Guangzhou Analysis and Testing Center, a third-party authoritative testing organization. The enzyme activity reached 8.3 million international units (8.3×10 < 6th power > U/g), and the specific activity purification reached 29 million international units (2.9× 10 < U/g. protein). Exceeding international standards and filling domestic gaps.
The company signed a technical R&D cooperation with Hong Kong Molecular Biology Laboratory (MBL.HK) to build a joint R&D center, and awarded the company the "protein and Enzyme Engineering International Technical Cooperation Project".
Plant SOD can effectively remove the garbage in the blood, and it is an efficient scavenger of free radicals in the body. It can catalyze the reduction of lipid peroxides, thus inhibiting the chain reaction of peroxide reaction and free radical removal, improving the utilization rate of oxygen, improving peripheral blood flow, reducing myocardial lipofuscin, obviously improving the activity of SOD in the body, resisting oxidation, preventing aging, inhibiting gene mutation, eliminating fatigue and delaying the aging process. It can be described as a scavenger of human garbage and a guardian of the first line of defense of the human body.
1. The decrease of SOD in human body induces the accumulation of oxygen free radicals and induces cardiovascular diseases: the continuous generation of oxygen free radicals will cross-link with protein in the vascular wall, which will thicken and harden the vascular wall of arterioles, narrow the lumen, increase the resistance in the blood vessels and make the blood viscous, thus causing cardiovascular diseases such as hypertension, arteriosclerosis, heartache, coronary heart disease, myocardial ischemia, cerebral hemorrhage and cerebral thrombosis. SOD can block the cross-linking reaction of blood vessel wall, and taking SOD products can prevent and treat cardiovascular diseases.
2. Acute emphysema caused by oxygen free radicals: Acute emphysema is the most common symptom of oxygen poisoning. This is due to lung dysfunction caused by hyperoxia tension. When high concentration oxygen enters the lung, alveolar macrophages are damaged first, releasing some factors that can activate polymorphonuclear leukocytes and neutrophils, which can produce a large number of reactive oxygen free radicals, damage bronchial endothelial cells, change the structure and basic functions of alveolar microvessels, and lead to lung injury and emphysema. SOD has antioxidant function, and taking SOD products can prevent and treat emphysema.
3. Oxygen free radicals cause autoimmune diseases: The oxidative damage of oxygen free radicals to protein and its cross-linking changes lead to the appearance of some foreign proteins, which trigger the immune allergic reaction of the body and form autoimmune diseases. Common diseases include rheumatoid arthritis, systemic lupus erythematosus, dermatomyositis, localized enteritis, nonspecific diseases (such as atypical pneumonia and atypical hepatitis) and acquired immune deficiency (such as AIDS). SOD can enhance immune function. SOD products have special effects on systemic immune diseases.
4. Relationship between diabetes and free radicals and SOD: Diabetes is a common endocrine and metabolic disease with genetic tendency, and its basic pathophysiology is the metabolic disorder of sugar, fat and protein caused by absolute and relative deficiency of insulin secretion. The activity of SOD in diabetic patients decreased significantly, and the plasma lipid peroxide and hydrogen peroxide increased significantly. After a long illness, the heart, blood vessels, eyes, kidneys and nerves are often complicated. Free radicals are involved in the occurrence and development of diabetes. SOD plays a very important role in the recovery of diabetes, so supplementing SOD in the treatment of diabetes has a positive effect on preventing complications and protecting islet β cells from secreting insulin.
5. Oxygen free radicals can lead to senile cataract and the formation of fibrous tissue behind lens: excessive reactive oxygen free radicals are produced in the eye, which causes the changes of protein in the eye, especially the cross-linking and denaturation of A- crystallin and B- crystallin. At the same time, it can also make the plasma membrane of lens undergo peroxidation reaction, form insoluble polymer products and gather in the middle of lens, so various enzymes lose their activity, leading to the formation of cataract. Supplementing SOD can prevent the formation of cataract.
6. Excessive accumulation of oxygen free radicals can induce cancer: free radicals can cause oxidation, destruction or cross-linking changes of DNA, denature nucleic acids, cause spatial changes and single-strand breaks of DNA, thus affecting the function of information transmission and the characteristics of transcription and replication, leading to the decline of protein's synthetic ability and synthetic errors, and finally leading to gene mutation and canceration. In addition, in the process of radiotherapy and chemotherapy, because both radioactive substances and chemical substances can induce excessive reactive oxygen radicals, radiotherapy and chemotherapy syndromes, such as alopecia, inflammation, urinary incontinence, hematuria, gastrointestinal dysfunction, spasm and so on, will inevitably occur. SOD can not only prevent the occurrence and development of cancer, but also reduce the negative effects of radiotherapy and chemotherapy.
7. Relationship between oxygen free radicals and skin diseases: The main cause of pigmentation (including wine thorn spots, senile spots, freckles, chloasma and pigmentation) is oxygen free radicals, which act on unsaturated fatty acids in the body to produce unstable lipid peroxides, and then produce aldehydes, especially malondialdehyde, which can quickly attack phospholipids and react with protein to form pigmentation. SOD can effectively remove oxygen free radicals, prevent pigmentation, and also remove pigmentation deposited in the skin.
8. Oxygen free radicals aggravate acute inflammation and edema: In the process of inflammatory infection, when neutrophils devour microorganisms, they produce a large number of reactive oxygen free radicals, which destroy the cell structure and further aggravate inflammation. Treatment with SOD products can relieve pain and fundamentally cure arthritis, scapulohumeral periarthritis and other symptoms.
9. Relationship between diseases of hepatobiliary system and free radicals: Lipid peroxidation of oxygen free radicals on biofilm causes damage to hepatocyte membrane, leading to degeneration and necrosis of hepatocytes, destroying liver tissue structure and damaging liver. SOD can reduce the level of toxin in blood, prevent hepatic coma, reduce blood ammonia, reduce the burden on liver and improve liver function, and has obvious curative effect on hepatitis, hepatomegaly, hepatic ascites, liver fibrosis and fatty liver. Supplementing SOD has a miraculous effect on hepatobiliary diseases.
10, the relationship between gastrointestinal diseases and free radicals: The current data show that the pathological process from common gastritis, atrophic gastritis, superficial gastritis to gastric cancer is actually a process of increased free radical deposition or content. SOD can specifically scavenge free radicals to relieve pain, prevent and treat syndromes caused by antibiotics, radiotherapy and chemotherapy, as well as colitis, diarrhea and constipation.
To sum up, with consumers' awareness of health and diseases in China, more and more consumers pay more attention to safe, healthy and effective health care methods. The development of high technology is endless, and the development of turf industry will not stop. Turf industry has brought brand-new reforms and different achievements to some traditional related industries, and has become one of the focuses of people's extensive attention. Call on the majority of scientific researchers, in the future application of SOD experiments, in addition to research, but also to study the relevant market, to clarify the mechanism. Only in this way, the application of SOD in China can continue to deepen, and it is possible to further improve to the international advanced level. Become the most brilliant page in the history of turf development in the world.