Why are all monoclonal antibodies from mice?

Because this technology was invented by mice, it was the technology with monoclonal antibodies before it was applied. Even when it was first invented, it was taken to the school's pattern office. People thought it was useless and didn't apply for a patent. Some of them are widely used now because they have no patents.

Immunizing animals is the process of immunizing mice with target antigen to produce sensitized B lymphocytes. Generally, female mice aged 6-8 weeks are selected and immunized according to the pre-established immunization plan. Antigen enters peripheral immune organs through blood circulation or lymphatic circulation, which stimulates the corresponding B lymphocytes to clone, activate, proliferate and differentiate into sensitized B lymphocytes.

Monoclonal antibody is a highly homologous antibody produced by a single B cell clone, which only targets a specific epitope. Usually prepared by hybridoma technology. Hybridoma antibody technology is based on cell fusion technology, which fuses sensitized B cells with the ability to secrete specific antibodies and myeloma cells with unlimited reproductive ability into B cell hybridoma.

Monoclonal antibodies are produced by artificially prepared hybridoma cells, which are fused by antigen-activated B cells and myeloma cells. The monoclonal antibody has high purity, high sensitivity, strong specificity, less cross reaction and low preparation cost. Disadvantages: there are certain requirements for technology, and it is easy to lose epitopes by chemical treatment of antigens.