Contents 1 Pinyin 2 English Reference 3 Overview 4 Medical tests for C-reactive protein 4.1 Test name 4.2 Classification 4.3 Principle of C-reactive protein measurement 4.4 Reagents 4.5 Operation methods 4.6 Normal values ??4.7 Clinical significance of test results 4.8 Notes 4.9 Related diseases 1 Pinyin
Cfǎn yìng dàn bái 2 English reference
Creactive protein 3 Overview
CRP is an acute phase response protein that can activate complement and promote Phagocytosis and other immune regulatory effects. Currently, immunochemical methods are mainly used to measure CRP, such as one-way immunodiffusion, rocket immunoelectrophoresis, latex agglutination, ELISA, etc. Compared with the rate scattering turbidity method, the latex agglutination test has high sensitivity (97.7%), specificity (98.04%) and accuracy (98%), and is simple, economical and fast. Although it is a qualitative test, the titer can be used The assay further yielded semi-quantitative results. Only latex agglutination test and ELISA assay are introduced here. 4 Medical examination of C-reactive protein 4.1 Test name
C-reactive protein 4.2 Classification
Blood biochemical examination> Protein determination
4.3 Principle of C-reactive protein measurement< /p>
The latex reagent is sensitized with purified anti-human CRP antibodies and can combine with each subunit of the CRP molecule in the patient's serum to produce agglutination. Clear agglomerated particles can appear after 1 minute by centrifugal precipitation. A positive result indicates that the CRP content in the patient's serum is >10 μg/ml. 4.4 Reagents
(1) CRP reagent (1ml).
(2) CRP diluent (50ml). 4.5 Operation method
Add 0.5ml of CRP diluent into the test tube, and then add 1 drop (50ul) of serum. Mix evenly, add 10ul of CRP reagent, mix and centrifuge (3000 rpm) for 1 minute, and observe the results with a microscope. Those with clear agglutination are considered positive; those without agglutination are considered negative.
(1) The reagent can be stored at 4 to 25°C, but cannot be frozen. Shake well before use. It should be operated strictly to prevent contamination as much as possible. Please refer to the kit instructions for details.
(2) It is suitable for the determination of body fluids such as serum, chest, ascites, cerebrospinal fluid, etc. However, the specimens should not be anticoagulated with oxalate anticoagulant.
(3) The biggest advantage of this reagent is that it is fast and simple, the reagent is stable and easy to store, and the serum does not need to be inactivated and is not subject to RF interference. 4.6 Normal value
Negative (normal human serum content <10μg/ml). 4.7 Clinical significance of laboratory results
CRP is an acute phase protein, and the normal human serum content is less than 10ug/ml. In cases of myocardial infarction, rheumatism, tissue damage, malignant tumors, surgical trauma, and various acute or chronic infections, CRP can increase rapidly after 4 to 6 hours; as the condition improves, it will quickly drop back to normal. C-reactive protein is positively correlated with the degree of tissue damage. It is not affected by other acute phase indicators (such as blood pressure, respiration, heart rate, etc.), nor is it directly affected by commonly used anti-inflammatory drugs or immunosuppressive drugs. Therefore, it can be used as one of the preferred indicators for observing the degree of acute inflammation, tissue damage and treatment effects. C-reactive protein in the serum of patients infected by microorganisms increased to varying degrees. The increase in C-reactive protein in patients with bacterial infection is more obvious than that in patients with viral infection, so it can be used as one of the differential diagnosis indicators of bacterial infection or viral infection. Suffering from acute or chronic rheumatism, there is a significant difference in the C-reactive protein content in the serum, which increases significantly in acute cases. In addition, detecting C-reactive protein can provide a basis for postoperative infection. C-reactive protein levels usually return to normal 3 to 5 days after surgery. If it persists or rises again, it indicates infection or thromboembolism. Chronic rheumatoid arthritis, systemic lupus erythematosus and other diseases.
C-reactive protein is also often elevated in the active phase, and can also be elevated in cirrhosis and chronic progressive hepatitis. If the C-reactive protein in the serum increases significantly during kidney transplantation, it indicates rejection after transplantation. In patients with myocardial infarction, this protein is also elevated. C-reactive protein increases in malignant tumors or metastases, but not in benign tumors. For patients with elevated C-reactive protein in leukemia, concurrent infection should be considered. 4.8 Notes
(1) The reagent can be stored at 4~25℃, but cannot be frozen. Shake well before use. It should be operated strictly to prevent contamination as much as possible. Please refer to the kit instructions for details.
(2) It is suitable for the determination of body fluids such as serum, chest, ascites, cerebrospinal fluid, etc. However, the specimens should not be anticoagulated with oxalate anticoagulants.
(3) The biggest advantage of this reagent is that it is fast and simple, the reagent is stable and easy to store, and the serum does not need to be inactivated and is not subject to RF interference. 4.9 Related diseases