Extraction methods: dynamic continuous overflow extraction, ultrasonic extraction, multifunctional extraction, supercritical extraction, separation, impurity removal, membrane filtration, separation and purification of resin combined with original chromatographic technology, and short-distance molecular distillation drying methods: freeze drying, dynamic belt drying and spray drying.
Application of 1 and macleaya cordata in preparation of pesticides
2. Macleaya cordata total alkaloid pharmaceutical preparation and its preparation method
3. Separation and preparation technology of high-purity sanguinarine and chelerythrine.
4. A macleaya cordata plant powder insecticide
5. A total alkaloid with anticancer activity and its preparation method.
6. Plant-derived total alkaloid composition and its pharmaceutical preparation
7. A topical macleaya cordata swelling tincture for treating skin itching.
A veterinary macleaya cordata injection with broad-spectrum effects of clearing away heat, detoxicating, detumescence, antibiosis, antivirus and anti-inflammation is prepared from macleaya cordata and andrographis paniculata by soaking in ethanol to extract effective components, mixing and compatibility, cold and hot treatment, precipitation and impurity removal, etc. It is a pure natural green veterinary drug product. Has effect of clearing away heat and toxic material, relieving swelling, resisting bacteria, virus and diminishing inflammation, and can be used for treating various livestock influenza, upper respiratory tract infection, pneumonia, pig lung disease, digestive tract infection, infectious gastroenteritis, diarrhea, dysentery, piglet dysentery, yellow dysentery, fever, virus infection, fowl cholera, chicken dysentery, etc. The invention not only gives full play to the compatibility advantage of traditional Chinese medicine multi-effect two-way treatment to adjust the compound effect, but also embodies the advantage of modern science and technology to refine Chinese medicine, and has the advantages of convenient use, wide application range, quick response, obvious curative effect, stable quality, no toxic or side effects, no residue and meeting the development requirements of current "green products".
Macleaya cordata has detoxification, detumescence and insecticidal effects. Modern pharmacological research shows that it is effective in treating various inflammations. As the main effective component of Macleaya cordata, sanguinarine plays an important role in the efficacy of Macleaya cordata. In vitro bacteriostatic test and veterinary clinical test showed that the minimum inhibitory concentration (MIc) of Macleaya cordata injection for Escherichia coli was 7.8 1ug/ml, and the total effective rate for treating yellow and white dysentery of piglets was 80%, which had the advantages of low dosage and low cost. Macleaya cordata injection has antibacterial and anti-inflammatory effects, and has a good therapeutic effect on yellow and white dysentery of piglets. It has been listed in the 96th edition of the quality standard for veterinary drugs of the Ministry of Agriculture. Rao Maoyang et al. (200 1) selected Macleaya cordata, Mahonia, Tripterygium wilfordii, Andrographis paniculata, Sophora flavescens and other pure Chinese herbal medicines as raw materials. The experiments showed that it had good therapeutic and preventive effects on common poultry diseases such as cholera, pullorum disease and typhoid dysentery, and the cure rates for 2,493 local hybrid chickens and ducks were 93.9% respectively.
The results showed that Macleaya cordata had killing effect on fly maggots. The LC50 of Macleaya cordata extracted by acidic ethanol to 3rd instar maggots was 148.75g/L, and the 95% confidence interval was122.19g/L ~180.94g/L ~ SJ. Gong Zhongfu et al. (2002) observed the insecticidal activity of crude methanol extracts from Macleaya cordata et al. 19 plants against urticaria in rabbits through in vitro insecticidal experiments. The results showed that 50% aqueous suspensions of Macleaya cordata, Stemona, Pericarpium Citri Tangerinae, Cortex Dictamni Radicis, Cortex Meliae, Radix Sophorae Flavescentis, and Crotalaria pigmentosa could kill adult rabbit urticaria within 4 hours, among which Macleaya cordata and Stemona could kill urticaria within 2 hours, and their activity was not less than 2mg/L avermectin aqueous solution.
3 Improve liver function and enhance immunity.
Macleaya cordata has a good immune enhancement effect and can stimulate the functions of T lymphocytes and B lymphocytes. Macleaya cordata has a good effect on improving liver function in acute liver injury caused by various drugs, which can significantly reduce serum lactate deaminase (LDH) level, reduce animal mortality, increase serum albumin/globulin (A/G) ratio, effectively protect hepatocyte membrane and inhibit liver fibrosis.
4 Anti-tumor effect
Sanguinarine exists in many plant extracts. Because of its extensive pharmacological effects, it has been used by American medicine and traditional medicine in North China for centuries. Sanguinarine is usually used as Chinese medicine to treat various diseases, including cancer. Duke (1985) found that sanguinarine has therapeutic effect on pancreatic cancer. Experiments show that sanguinarine can significantly inhibit the growth and development of pancreatic cancer cells ASPC- 1 and BxPC-3 and their colony-forming ability. Haseeb Ahsan(2007) also confirmed that sanguinarine can induce apoptosis of human pancreatic cancer cells AsPC- 1 and BxPC-3 by regulating Bcl-2 protein. ℃hang et al. (2007) found that compared with the control group, the groups added with 2pM and 3pM sanguinarine showed toxic effects on KB cancer cells after 24 hours of exposure, and the MTT decreased to 83% respectively. Sanguinarine can also inhibit the colony formation and growth ability of KB cancer cells. In addition, the addition of 2pM and 3uM sanguinarine obviously induced the apoptosis of KB cells. K 1735-M2 cells are melanoma cells and are considered as a good model of metastatic melanoma. Teresa L et al. (2008) found that sanguinarine can kill K 1735-M2 cells and hinder their proliferation. Holy J et al. (2006) proved that sanguinarine inhibits breast cancer by cleaving the cytoplasmic transport of cyclin in MCF-7 cells. Wee Xin Hui Cheryl et al. (200 1) found that sanguinarine can inhibit the proliferation of sMM C-772 1 human hepatoma cells and has obvious killing effect on hepatoma cells. Lee B et al. (2008) showed that sanguinarine can regulate vascular smooth muscle. Wu et al. (2008) found that Macleaya cordata has effects on the activities of Ca2_, Mg2+-ATPase and SDH in rat myocardium, which can induce myocardial apoptosis, while sanguinarine in Macleaya cordata can affect the flow of Ca2+, and both protein kinase and Ca2+ participate in the signal transduction of apoptosis and regulate apoptosis. In addition, sanguinarine can also inhibit cholinesterase activity, strengthen heart activity, stimulate saliva secretion, and has diuretic and peripheral anti-adrenergic sympathetic effects.
Macleaya cordata R.br, also known as trumpet grass, trumpet stem, paulownia stem, Bolehui, Erythrina corniculatum, mint, Veronica flavescens, San Qian III, wild hemp seed and cupping, is a tall perennial herb of Macleaya of Papaveraceae, which is used to treat scabies and furuncles. The earliest herbal literature record in Macleaya cordata is Notes on Materia Medica. Now named Macleaya cordata, it is widely used as raw materials for medicine, biological pesticides, veterinary drugs and fine chemical products.
The main chemical component of Macleaya cordata is alkaloid, from which sanguinarine, chelerythrine, protopine, α-allocryptopine and β-allocryptopine are separated. The effective chemical components in Macleaya cordata are mainly sanguinarine and chelerythrine, with the highest content in fruits.
The chemical constituents of Macleaya cordata include alkaloids sanguinarine, chelerythrine, protopine, α-allocryptopine, β-allocryptopine and Macleaya cordata. Namely chelerythrine, sanguinarine, sanguinarine, dehydrocorydalis, macleaya cordata, berberine, coptisine and salbutamol. The basic research on other polysaccharides, amino acids and inorganic elements shows that Macleaya cordata can be widely used in veterinary drugs, feed additives, medicines and cosmetics.
Modern research shows that Macleaya cordata has strong bactericidal and bacteriostatic activities, as well as good anti-inflammatory and antiarrhythmic effects. Modern clinical medicine has a wide range, which can treat trichomoniasis, pneumonia in children, acute tonsillitis, bronchopneumonia, subauricular adenitis, acute appendicitis, deep abscess, cholecystitis, vasculitis, cervical erosion, incomplete intestinal obstruction and so on. .
Sanguinarine is a feed additive announced by People's Republic of China (PRC) and the Ministry of Agriculture No.318. At present, there is no production unit that can produce and supply this product in China.
Macleaya cordata injection is a kind of veterinary drug recorded in the quality standard of veterinary drugs of the Ministry of Agriculture (2003 edition). At present, there are hundreds of veterinary drug factories in China.
The harm of chemical pesticides to people and the environment has prompted the pesticide industry to change to the development direction of new environmentally friendly pesticides, and the development of plant-derived pesticides is one of them. However, plant-derived products, especially plant extracts, are not very successful in developing plant pesticides because of their own characteristics. The total alkaloids of Macleaya cordata are one of the few successful products developed from plant extracts into botanical insecticide. The total alkaloid extract of Macleaya cordata produced by the company completely meets the pesticide quality standard of Macleaya cordata extract registered by EPA in the United States. This product is mainly used in European and American countries to prevent insects and bacteria in the early stage of picking vegetables and fruits, and belongs to the category of green plant pesticides. 0.5- 1% dosage.
In the field of medicine and oral care products, the application of sanguinarine and chelerythrine in Macleaya cordata is also expanding. In addition, α -allocryptopine in Macleaya cordata has strong anticancer physiological activity and is currently in the research and development stage. The total alkaloids of Macleaya cordata and their derivatives have a very broad development and application space.
Industrialization direction of total alkaloids of Macleaya cordata 60% total alkaloids veterinary drug Macleaya cordata injection raw material pesticide (botanical insecticide) sanguinarine (98%) feed additive Bojing spray body surface disinfection Bozu foot washing powder prevention and treatment of tinea pedis α-allocryptopine anticancer sanguinarine oral care product Tan Shixun Tom tan.
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