Cepharanthine is a dichotomous valine alkaloid isolated from cepharanthine and Fritillaria cirrhosa. Has antitumor, antimalarial, antibacterial and immune function regulating effects. Modern research suggests that it can also stimulate reticuloendothelial system, activate hematopoietic tissue and promote the proliferation of bone marrow tissue. Clinically, it has been used to prevent and treat leukopenia caused by radiotherapy, chemotherapy and other reasons, and has received certain results. Therefore, cepharanthine is considered as a low toxic and effective leukopenia drug.
Stemona can partially reverse the multidrug resistance of leukemia cells, and the effect is obviously better than that of verapamil, and the cytotoxicity is much less than that of verapamil when the dose is reversed. Foreign studies also show that cepharanthine has a good reversal effect on many drug-resistant cells. Stemona root can enhance the immune function of cells and macrophages in vivo. In Japan, cepharanthine has been used as a leukocytosis drug for more than a year, and there is no obvious toxic side effect.
Production method:
1.？ Take 1kg rehmannia root powder, moisten it with 0.6L 5% Na2CO3 solution, let it stand for 2 hours, and air dry it.
2. Make the plant tissue fully absorb the lye. Then soak in benzene 1.5L for 3 days, then filter, and soak the residue in benzene for 2 days.
3. After filtering, soak in the same amount of benzene once, collect and combine the benzene solutions for three times, and extract with 10% hydrochloric acid. A large amount of rice congee is precipitated in the process of acid leaching, which is easy to emulsify.
4. Extract benzene solution with acid until it contains no alkaloid, alkalize the extracted acid solution with saturated Na2CO3 solution, extract the alkalized solution with chloroform for four times, and dry the combined chloroform extracts with anhydrous sodium sulfate. Chloroform was recovered by distillation to obtain light yellow foam total alkaloids, weighing about 65438±07.5g g.
5. Dissolve the total alkaloids in 35ml acetone, let it stand for 2 hours, and white needle-like crystals can be separated out. Filter, wash 65438 0 times with a small amount of acetone, and dry to obtain 65438±0.8g?
6. Add 2.5 ml of benzene to the acetone mother liquor, leave it overnight, separate out columnar crystals, filter, wash with acetone again, and dry to obtain 6.8 g of cepharanthine benzene adduct. Dissolving it in chloroform, passing through alumina chromatography column (1g alkaloid is 3g alumina), concentrating, filtering and drying the obtained precipitate to obtain yellowish foam amorphous powder, namely cepharanthine.