In China National Intellectual Property Administration, China, in addition to Nanjing Liangyoupeijiu Seed Industry Co., Ltd., other units and individuals have published more than 200 patent documents of hybrid rice invention technology around the world. Among them, there are few foreign applications, mainly from the United States and Japan. For example, the document CN9 1 102598.7 of American Peripheral Products Company relates to a method for producing hybrid rice by using perennial male sterile rice plants. The document CN88 1092 18.5 of Japanese company relates to a method for cultivating hybrid rice seeds. It includes making male sterile female parent rice resistant to herbicide, crossing female parent rice with male parent rice to produce hybrid rice seeds, and treating parents with herbicide resistant to female parent rice to kill only male parent rice, thus selecting hybrid rice seeds. The document CN0 18 16569 jointly applied by two Japanese companies relates to a detection method for restoring BT cytoplasmic male sterility gene in hybrid rice breeding. It can use several PCR markers near Rf- 1 site and the linkage between Rf- 1 site to detect Rf- 1 gene.
China submitted the most patent applications for hybrid rice. Among them, there are about 56 individual applications, and some patented technologies owned by natural persons have broad application prospects and have been promoted by private high-tech enterprises. The application of research institutions is about 135. The main institutions applying for scientific research are Rice Research Institute of Guangdong Academy of Agricultural Sciences, Hunan Plant Protection Research Center, Hunan Hybrid Rice Research Center, South China Agricultural University, Nanjing Agricultural University, Jiangsu Academy of Agricultural Sciences, and Crop Research Institute of Sichuan Academy of Agricultural Sciences. There are few applications from enterprises, among which Dabeinong and other companies have the strongest patent strength. Dabeinong Group, as the holding company of Nanjing Liangyoupeijiu Seed Industry Co., Ltd., is one of the largest agricultural companies in China. Among the national agricultural enterprises, Dabeinong Group has the strongest scientific research strength. In terms of market layout, it is the only local enterprise in China that may compete with foreign agricultural enterprise groups such as Monsanto. The hope of agriculture in China depends on private scientific and technological enterprise groups such as Dabeinong.
In situ hybridization)
It is a molecular biological method that uses labeled nucleic acid probes, non-radioactive detection systems or autoradiography systems to qualitatively, locate and relatively quantitatively study nucleic acids in tissue sections, cell smears and chromosome sections, and has the advantages of sensitivity, specificity and intuition. It has gradually become one of the commonly used techniques in molecular biology and molecular pathology, and is widely used in tumor biology, hematology, genetics, microbiology, cell and molecular biology, neuroendocrinology, immunology and other fields, as well as treatment evaluation and prognosis judgment. In-situ molecular hybridization of colonies: refers to printing transformed or infected colonies on a filter membrane, cracking with alkali, washing and drying after neutralization, and then using radioactive DNA or mRNA of the target gene as a probe for hybridization autoradiography. Any colony with black spots should be selected.