Product information:
SRT SEC of Chengdu Moore Scientific Instrument Co., Ltd. is a molecular exclusion chromatographic column filled with hydrophilic modified silica gel, which is specially designed for efficient separation of polysaccharide, protein and nucleic acid.
Chemical modification technology ensures good reproducibility between chromatographic columns. The hydrophilic coating on the surface of silica gel is completely covered, which makes the chromatographic column have excellent stability and durability.
Two chromatographic columns, SRTSEC-300 and SRTSEC-500, are connected in series, which completely meet the next molecular weight and fraction of heparin sodium in China Pharmacopoeia (20 15 edition).
Requirements for testing quantum distribution. The cubic force is fitted by GPC software, and the correlation coefficient of the calibration curve is 0.9999, which can be used to calculate the molecular weight distribution of heparin sodium.
Make an accurate judgment.
Column specifications:
chromatographic column
SRT SEC-500 precolumn SRT SEC-500 analytical column SRT SEC-300 analytical column
standard
7.8X50mm 7.8X300mm 7.8X300mm
Art numbering
2 15500-78052 15500-78302 15300-7830
Test data of molecular weight and molecular weight distribution of heparin sodium;
Solution preparation:
Test solution: take a proper amount of heparin sodium sample, add mobile phase to dissolve it, and dilute it into a solution with a concentration of 5mg/mL.
Reference substance solution: Take a proper amount of heparin molecular weight reference substance, add mobile phase to dissolve and dilute it, and make a solution with a concentration of 5mg/mL.
Systematic adaptive solution: Take a proper amount of heparin sodium molecular weight systematic adaptive reference substance, add mobile phase to dissolve and dilute it, and make a solution with a concentration of 5mg/mL.
Column requirements:
According to exclusion chromatography, hydrophilic modified bonded silica gel was used as filler.
Pre-column, SRT sec-500
Macrochromatographic conditions:
Mobile phase: 0. 1 mol/l acetic acid solution.
Column temperature: 30℃
Detector: differential
Sample volume: 25 microliters
Brewing attempt spectrum and result evaluation;
Fitting curve:
Test results of system suitability solution and heparin sodium samples of Chengdu Moore Scientific Instrument Co., Ltd.;