Pseudouridine generally refers to pseudouridine, which is the most abundant modified nucleoside in non-coding RNA and a natural structural analog of uridine. Among them, ribose is not linked to uracil N 1, but to C5 of pyrimidine ring, which is a rare nucleotide in tRNA.
Pseudouridine, as the most abundant modified nucleotide in RNA, is ubiquitous and dynamically regulated in human mRNA. Pseudouridylation of artificial RNA has been proved to affect the interaction between RNA and RNA- protein, so it can be assumed that human pus may be able to pseudouridylate new mRNA precursors, thus playing a role in the processing of nuclear mRNA precursors.
It is found that using pseudouridine instead of uridine can significantly reduce the immunogenicity of mRNA transcribed in vitro, improve the stability and expression intensity of mRNA, and is widely used in nucleic acid drugs.