Sedative (1) mice were injected with 0.2 17ml/kg and 0. 109ml/kg of volatile oil from Korean pine leaves, which could obviously reduce the sleep of mice with spontaneous activity and subthreshold dose of pentobarbital sodium.
(2) Effect on body temperature After intraperitoneal injection of Korean pine leaf oil in mice, the normal body temperature can be reduced, and its cooling effect will gradually weaken with time. Intraperitoneal injection of Korean pine leaf oil 0. 1.09 ml/kg has a very significant antipyretic effect on the body temperature of fever rats caused by dry beer yeast powder.
(3) Analgesic effect: 0.2 17ml/kg Korean pine leaf oil can obviously prolong the pain reaction time of mice (hot plate method), and 0.2 17ml/kg Korean pine leaf oil and 4.34ml/kg Korean pine leaf oil can obviously inhibit the writhing reaction of mice caused by acetic acid.
3. Anti-inflammatory effect: 0.2 17ml/kg and 0. 109ml/kg of Korean pine leaf oil were injected intraperitoneally and 4.34ml/kg was taken orally, which obviously inhibited the swelling of rats' feet caused by carrageenan and mice's ears caused by xylene.
13. Hypolipidemic effect: Pine needle water extract (5g/kg, 10g/kg) can significantly regulate the blood lipid of hyperlipidemia mice induced by egg yolk milk and hyperlipidemia rats induced by high-fat feeding, significantly reduce total cholesterol and relatively increase high-density lipoprotein cholesterol.
3. Anti-aging effect The survival test of Drosophila showed that the water extract of pine needles could significantly prolong the life span of Drosophila adults at the concentration of 1.0%-4.0%, and also had the effects of resisting gene mutation and DNA damage, improving the activity of superoxide dismutase (SOD) in red blood cells and inhibiting the activity of monoamine oxidase B (MAO-B) in brain tissue of mice. In addition, pine leaves also have the function of dissolving aging substances in human body, regulating and promoting the function of body and tissue, and improving human health.
⒌ Antiviral effect Observed by tissue culture technology, the water extract and alcohol extract of Pinus massoniana (containing crude drug 10g/ml) have anti-herpes simplex virus type I, and the water extract of Pinus massoniana (300μg/50μl) has anti-hepatitis B virus surface antigen.
6. Other functions The contents of α-pinene, myrcene and limonene in Korean pine and Pinus massoniana are high, which have obvious antitussive and expectorant effects and strong antibacterial effects. The decoction of pine needles and amino acid extracts were given to mice by gavage, with 5.34g crude drug/10g per day, for a total of 1 week. The results show that the appetite of mice is obviously enhanced, the activity is increased and the coat color is bright.
The LD of toxic Korean pine leaf oil injected intraperitoneally in mice was 2.65438 0.028 ml/kg. 1, cure foot weakness and 12 winds, joint pain can't be done: 60 kg of pine leaves, finely cut, boiled in four Shi Shui, boiled in four buckets and nine liters to brew five buckets of rice, as usual; Don't boil pine leaf juice to dye rice for food. Fill your head with mud and deliver it on the seventh day. You get drunk after drinking it. ("Qian Jin Fang" pine leaf wine)
2, treatment of low back pain: one or two pieces of Pinus massoniana leaves, decoction to slag, add rock sugar one or two to adjust clothes. ("Jiangxi folk prescription")
3, wind treatment: 30 pounds of pine leaves, two stones and five fights of wine, three or seven days of stains, one service, five or six degrees. (Qian Jinfang)
4, cure swelling and pain: mountain pine must soak in wine; Add clams to the residue and pat it on the affected area. (Essentials of Raw Herbs)
5, treatment of falls and injuries: young leaves of Pinus massoniana branches, baked and ground into very fine powder. Take it twice a day, one yuan each time, send warm sweet wine. ("Jiangxi folk prescription")
6, treatment of bruises, sprains, skin itching, lacquer sores, eczema: fresh pine leaves decoction fumigation, even washing several times. (Common Folk Herbs in Zhejiang)
7, cure rheumatism stubborn tinea: send out (fry black) one or two, calomel, camphor one San Qian each. Wet is dry, dry is mixed with oil, and rice vinegar is mixed if it itches. And treat chilblain. (authentic surgery)
8. Treating wind sores, experiencing wind pain, soft feet and paralysis: Tricholoma matsutake is used to mash and bake new people. Every time, use Tricholoma matsutake, Lycium barbarum, soak in wine, take it regularly, and stay drunk for a long time. (authentic surgery)
9, cure headaches; Fresh Tricholoma matsutake, mashed, roasted, soaked in wine and often drunk; Take out the residue, stick it on the top door, and wrap it in cloth for the first three days. (Authentic Fang Mai)
10, the stroke treatment is remote, the teeth are urgent, and the tongue can't turn: one catty of pine leaves, mashed juice, a bucket of sake for two nights, near the fire for one night, taking half a liter for the first time, gradually increasing to one liter, and sweating on the head and face will stop. (Qian Jinfang)
1 1, treatment of insomnia, vitamin c deficiency, nutritional edema: one or two fresh pine leaves, decocted in water. (Common Folk Herbs in Zhejiang)
12, treatment of wind tooth swelling and pain: one hold of pine leaves, one combination of salt and two liters of wine. Decoct and rinse. (Sheng Huifang)
13, the treatment of scrotum wet itching: Songmao decoction wash frequently. ("purely unilateral")
14, prevention of hookworm disease: pine needles in proper amount, decocted in water into thick juice, and wiped feet and calves before going barefoot. Selected works of unilateral new medical treatment in Xuzhou 1. Materia Medica: Loose hair, dispel wind and dampness, and treat tinea and malignant sores. The dryness is beneficial, frying black is good at removing rheumatism, stubborn tinea is wet and rotten, soaking is not dry, and it is suitable for frostbite in winter. Pouring raw materials into pills can cure diseases caused by strong wind, or wind pain, or beriberi flaccidity, or headache caused by head wind. The above diseases are suitable for patients with rheumatism, and are prohibited if they are blood deficiency and wind dryness. 2. "Records of Famous Doctors": Treating rheumatic sores, promoting hair growth and calming the five internal organs.
[13] Rihuazi Materia Medica: chilblain and rheumatic sores.
4. Compendium of Materia Medica: Expelling wind, foot pain and joint pain, killing Mi Chong.
⒌ "Raw Herbal Essence": killing scorpions, drying water, relieving itching, burying mouth (healing sore), washing scabies and treating scabies.
[6] "Medical Mirror": Breaking the plague.
⒎ Handbook of Common Chinese Herbal Medicines for Guang Jun: Treating neurasthenia, vitamin C deficiency and nutritional edema; Prevention and treatment of meningitis and influenza.