1967, Egger and Petran successfully made an optical cross section with a * * * focusing microscope.
1977, Sheppard and Wilson first described the nonlinear relationship between light and atoms of the irradiated object and the Raman spectrum of the laser scanner.
1984, Biorad introduced the world's first commercial * * * focusing microscope, the model is SOM- 100, and the scanning mode is step scanning.
1986 MRC-500 is improved to beam scanning, which is used as the * * * focusing system of biological fluorescence microscope.
1987, White and Amos published the article "The coming of the era of focusing microscope" in the British journal Nature, which indicated that LSCM has become an important tool for scientific research.
Subsequently, Zeiss, Leica, Meridian, Olympus and other companies have successively developed different models of * * * focusing microscopes, and the product performance has been continuously improved and updated, and the application scope has become wider and wider.