be entitled to
ApplicationNo.: 2005 10005038.9 Application Date: 2005-0 1-3 1.
The invention discloses a nattokinase and its coding gene and application, aiming at providing a nattokinase and its coding gene and its application in the production of nattokinase. The nattokinase is protein with one of the following amino acid residue sequences: 1) SEQ ID №:1in the sequence table; 2) protein with fibrinolytic activity by substituting, deleting or adding one to ten amino acid residues in the amino acid residue sequence of SEQNo.: 1 in the sequence table. Its expression method is to construct an Escherichia coli secretory expression vector containing nattokinase gene, introduce the constructed Escherichia coli secretory expression vector into Escherichia coli to obtain recombinant Escherichia coli, and induce the recombinant Escherichia coli to express nattokinase gene. The expression product of the invention has the advantages of high fibrinolytic activity, easy purification and good stability, which lays a foundation for the development and production of nattokinase as a new oral thrombolytic drug and has broad industrial application prospects.
Applicant: Institute of Microbiology, China Academy of Sciences.
Address: No.1 Zhongguancun North Street 100080 13, Haidian District, Beijing.
Inventors (designers): Jin Zhong, Liang Xiaobo, Jia Shifang, Sun Yufang, Chen Meiling, Chen Xiuzhu.